一株解钾高活性胶质芽孢杆菌的筛选与育种

Screening and Breeding of a Bacillus mucilaginosus Strain with Strong High Ability of Potassium Releasing

从钾页岩矿区分离筛选到一株解钾能力较强的野生型菌株,经鉴定并定名为胶质芽孢杆菌（Bacillus mucilaginosus HSC）。为了更好地适应生产和应用需求,对其进行了紫外诱变和N14＋离子束注入诱变处理,获得胶质芽孢杆菌诱变株（Bacillus mucilaginosus HSCUP-76-8）。该菌株生产性状与应用性状良好,具有生长速率快、发酵周期短（发酵时间为36~48 h）、菌体浓度大（稳定期细胞数可达2.4×109cfu/m L）、芽孢浓度可达2.0×109cfu/m L、芽孢转化率高（接近83%）、解钾能力强（2.8μg/m L）等生理学特性,为钾页岩菌肥的开发与生产提供了新的菌种资源。

In this study, a wild type strain with strong ability of potassium releasing was isolated from potassium shale mining area, which was identified and named Bacillus mucilaginosus HSC. In order to make it better adapted to the production and application requirements, Bacillus mucilaginosus HSC was treated by UV mutagenesis and N14＋ ion implantation, obtaining Bacillus mucilaginosus HSCUP-76-8. This strain has good production traits and application character, such as the fast growth rate, short fermentation period （36--48 hours）, high thalli concentration（the number of stationary phase ceils can reach up to 2.4 × 109 cfu/mL）, spore concentration can reach up to 2.0 × 109 cfu/mL, high spore conversion rate （nearly 83%）, strong potassium releasing ability （2.8 μg/mL） and so on. This research will provide new strain resources for the development and production of shale potassium bacterial fertilizer.