摘要
目的:探讨长链非编码RNA HOX转录反义RNA(HOX transcript antisense RNA,HOTAIR)在宫颈癌中的表达及对宫颈癌He La细胞增殖和凋亡的影响。方法:实时荧光定量PCR法检测36例宫颈癌组织及其相应癌旁组织中HOTAIR mRNA的表达,分析其与宫颈癌患者临床病理特征之间的关系;将靶向HOTAIR基因的短发夹RNA(short hairpin RNA,shRNA)慢病毒液感染人宫颈癌HeLa细胞后,应用CCK-8(cell counting kit-8)法和FCM法分别检测细胞增殖、细胞周期和细胞凋亡的情况。结果:宫颈癌组织中HOTAIR mRNA的表达水平明显高于其相应的癌旁组织(P<0.001);HOTAIR的表达与患者的临床分期、肿瘤大小和淋巴结转移有关(P值均<0.05),而与患者的年龄、组织学类型、分化水平、鳞状细胞癌相关抗原和宫体转移无关(P值均>0.05)。干扰HOTAIR的表达后,HeLa细胞的增殖受到抑制(P<0.05),G0/G1期细胞比率和细胞凋亡率增加(P<0.05,P<0.01)。结论:HOTAIR在宫颈癌组织中表达上调;干扰HOTAIR的表达可抑制HeLa细胞的增殖,并促进其凋亡。
Objective： To investigate the expression of long non-coding RNA HOX transcript antisense RNA（HOTAIR） in cervical cancer and its effect on proliferation and apoptosis of HeLa cells.Methods： The expressions of HOTAIR mRNA in 36 cervical cancer tissues and matched para-cancerous tissues were measured by real-time fluorescent quantitative-PCR, and its correlation with clinicopathological parameters was analyzed. After HeLa cells infected with lentivirus containing HOTAIR-targeted short hairpin RNA（shRNA）, the proliferation, cell cycle distribution and apoptosis were detected by cell counting kit-8（CCK-8） and flow cytometry（FCM）, respectively.Results： The expression level of HOTAIR mRNA in cervical cancer tissues was higher than that in para-cancerous tissues（P 〈 0.001）. The HOTAIR expression was correlated with clinical stage, tumor size and lymph node metastasis（all P 〈 0.05）, but it was not correlated with age, histologic type, differentiation, squamous cell carcinoma antigen（SCC-Ag） and uterine corpus invasion（all P 〉 0.05）. After knock down of HOTAIR, the proliferation of HeLa cells was inhibited（P 〈 0.05）, and the ratio of phase G0/G1 and the apoptosis of HeLa cells were increased（P 〈 0.05, P 〈 0.01）.Conclusion： The expression level of HOTAIR in cervical cancer tissues is up-regulated.Knockdown of HOTAIR can inhibit the proliferation of HeLa cells and promote the apoptosis.
出处
《肿瘤》
CAS
CSCD
北大核心
2015年第4期446-452,共7页
Tumor
