摘要
目的建立高效液相色谱法(HPLC)测定新肾炎胶囊中蒽醌类化合物大黄酸、大黄素、大黄酚的含量。方法采用Phenomenex C18柱(4.6 mm×250 mm,5μm),以甲醇-1%醋酸(70∶30)为流动相,检测波长254 nm,柱温25℃;流速1 m L·min-1。结果大黄酸、大黄素、大黄酚检测浓度分别在4.96~24.80μg·m L-1(r=0.999 6)、6.58~32.91μg·m L-1(r=0.999 9)、15.11~75.55μg·m L-1(r=0.999 9)范围内与峰面积呈良好的线性关系,平均加样回收率分别为100.78%,98.13%,99.29%。结论制剂制备工艺稳定,建立的含量测定方法简便、可靠,可用于制定主要治疗成分大黄素、大黄酚的含量下限以及非主要治疗成分大黄酸的含量上限,进一步保障制剂安全。
Objective To establish an HPLC method for the determination of anthraquinones including rhein, emodin and chrysophanal in xinshenyan capsules. Methods Anthraquinones were determined by HPLC with Phenomenex-C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and methanol-1% acetic acid (70 : 30) as the mobile phase. The flow rate was 1.0 mL· min-1 and the detection wavelength was set at 254 rim. Results The liner range of rhein, emodin and chrysophanol was 4.96-24.80μg· mL-1(r=0. 999 6), 6.58-32.91 μg · mL-1(r=0. 999 9) , and 15.11-75.55 μg · mL-1 (r=0.999 9) ,respectively, and the average recovery was 100.78%, 98.13% and 99.29% , respectively. Conclusion The method is simple and practical, the result is accurate and reliable and it can be used to determine the contents of rhein, emodin and chrysophanol in xinshenyan capsules.
出处
《医药导报》
CAS
2015年第4期509-511,共3页
Herald of Medicine
基金
全军医疗机构制剂标准提高科研专项课题重点项目(13ZJZ13)
关键词
新肾炎胶囊
大黄酸
大黄素
大黄酚
含量测定
色谱法
高效液相
Xinshenyan capsules
Rhein
Emodin
Chrysophanol
Content determination
Chromatography, highperformance liquid