CD14启动子基因多态性与中国高原藏族人群胃癌易感性研究

Study on the susceptivity and polymorphism of CD14 promotor with gastric cancer in Chinese Tibetan population

目的:研究分化抗原簇14(cluster of differentiation 14,CD14)启动子基因多态性与青藏高原藏族人群胃癌易感性的相关性。方法:采用聚合酶链反应-连接酶反应(PCR-LDR)技术对186例藏族胃癌患者和216例藏族正常对照人群CD14启动子基因多态性进行分析,并就特定基因型与胃癌发病风险及生存进行分析。结果:在胃癌组和对照组中,CD14 rs5744455C>T位点的等位基因T的频率分别是40.8%和20.0%,基因型TT的频率分别是20.0%和1.8%,等位基T和基因型TT增加了胃癌的发病风险;而rs59568848 C>T位点的基因型CT频率分别是34.5%和74.5%,CT明显降低了胃癌的发病风险,胃癌组基因型TT和等位基因C、T的频率与对照组之间无明显差异。结论:CD14启动子区rs5744455 C/T等位基因T和基因型TT明显增加藏族人群胃癌的风险,而rs59568848C>T中基因型CT则明显减少胃癌的发生。

Objective： To investigate the association between gastric cancer and the polymorphism of cluster of differentiation 14（ CD14） to find susceptibility genes and protective genes in Chinese Tibetan population from Tibet plateau. Methods： The CD14 between Tibetan patients with gastric cancer（ n = 186） and Tibetan control population（ n = 216） were detected by polymerase chain reaction with ligase detection reaction（ PCR-LDR）.Association of genotypes with the risk of gastric cancer was analyzed. Results： The genotypic frequency of allele T on CD14 rs5744455 C 〉T in patient groups and control groups were 40. 8% and 20. 0%,respectively,meanwhile the frequency of genetype TT in both groups were 20. 0% and 1. 8%,indicating that both allele T and genetype TT significantly reduced the risk of gastric cancer in Tibetan. Statistical analysis also revealed significantly decreased frequencies of genetype CT on CD14 rs59568848 C / T（ 34. 5% vs 74. 5%,P〈0. 001,OR = 0. 10,95% CI： 0. 03-0. 31） in patients with gastric cancer when compared with control group. Conclusion： Both allele T and genetype TT of CD14 rs5744455 C 〉T increase the risk of gastric cancer whereas genetype CT of CD14 rs59568848 C / T might contribute to protection in Chinese Tibetan population.