华南地区猪场环境中蔬菜细菌携带耐药基因情况调查

An investigation of resistance genes in bacteria from vegetables adjacent to the swine farms in Southern China

【目的】了解华南地区猪场环境中蔬菜样品附生细菌和内生细菌的耐药基因携带情况.【方法】采用PCR结合测序的方法检测氨基糖苷类、四环素类、β-内酰胺类、喹诺酮类、大环内酯类和林可霉素类耐药基因的携带情况.【结果和结论】四环素类耐药基因的分布范围比较广,附生细菌和内生细菌中此类耐药基因的场区检出率均为90%(9/10);蔬菜样品附生细菌和内生细菌所含的耐药基因是有差别的,而且附生细菌中的耐药基因比内生细菌的丰富,A、B、C、D、E、F、G、H、I和J样品附生细菌耐药基因的检出率分别是77.78%(21/27)、92.59%(25/27)、92.59%(25/27)、51.85%(14/27)、33.33%(9/27)、92.59%(25/27)、77.78%(21/27)、7.41%(2/27)、81.48%(22/27)和77.78%(21/27),内生细菌耐药基因的检出率分别是0(0/27)、11.11%(3/27)、11.11%(3/27)、11.11%(3/27)、29.63%(8/27)、48.15%(13/27)、29.63%(8/27)、33.33%(9/27)、25.93%(7/27)和22.22%(6/27).表明蔬菜中细菌的耐药情况,尤其是附生细菌的耐药情况可能已经非常严重,长此以往甚至会抑制作物生长,并且通过食物链危及动物和人体健康,应引起足够的重视.

【Objective】This experiment was conducted to investigate the resistance genes in bacteria from vegetable samples adjacent to the swine farms in Southern China. 【Method】PCR and sequencing were performed to detect the resistance genes of aminoglycoside,tetracycline,beta lactamase,quinolone,macrolide and clindamycin. 【Result and conclusion】Tetracycline resistance genes had a wide distribution range( the ground detectable rate of this kind of gene was 90%( 9 /10) both in accessory bacteria and endophytic bacteria). There were different resistance genes between accessory bacteria and endophytic bacteria from vegetables,and accessory bacteria had richer resistance genes than endophytic bacteria.The detectable rates of resistance genes in accessory bacteria of A,B,C,D,E,F,G,H,I and J samples were 77. 78%( 21 /27),92. 59%( 25 /27),92. 59%( 25 /27),51. 85%( 14 /27),33. 33%( 9 /27),92. 59%( 25 /27),77. 78%( 21 /27),7. 41%( 2 /27),81. 48%( 22 /27) and 77. 78%( 21 /27),respectively; the detectable rates of resistance genes in endophytic bacteria were 0( 0 /27),11. 11%( 3 /27),11. 11%( 3 /27),11. 11%( 3 /27),29. 63%( 8 /27),48. 15%( 13 /27),29. 63%( 8 /27),33. 33%( 9 /27),25. 93%( 7 /27) and 22. 22%( 6 /27),respectively. The results indicatethat the resistance problem in bacteria,especially accessory bacteria from vegetables,is probably very serious. It can even inhibit the growth of crops and threaten animal and human health through food chain to which enough importance should be attached.