HPLC-ELSD法测定消癌平注射液中牛奶菜醇和果糖含量

Determination of Condiritul and Fructose in Xiaoaiping Injection by HPLC-ELSD

目的:建立HPLC-ELSD法测定消癌平注射液中牛奶菜醇和果糖的含量。方法:采用COSMOSIL Sugar-D糖分析专用柱(4.6 mm×250 mm,5μm),乙腈-水(80∶20)为流动相,ELSD漂移管温度:100℃,氮气流量:2.5 L·min-1。结果与结论:牛奶菜醇和果糖在标准曲线范围内有较好的线性关系(r>0.9994)。本法简便、准确,为消癌平注射液中大极性成分的质量控制提供了依据。

Objective: To establish a quantitative HPLC-ELSD method for the determination of condiritul and fructose in Xiaoaiping Injection. Methods: A column of Cosmosil Sugar-D(4.6 mm×250 mm, 5 μm) was used with the mobile phase of water and acetonitrile(20∶80), the temperature of drift tube was set at 100 ℃ and the nitrogen f Iow rate at 2.5 L·min-1. Results and Conclusion: Calibration curves presented good linear regression(r>0.9994).The established HPLC-ELSD method is simple and suitable for the quality control of Xiaoaiping Injection.