高压氧干预对去卵巢大鼠骨质疏松的影响

Effect of hyperbaric oxygen on osteoporosis in rats with premature ovary failure

目的 观察高压氧干预对去卵巢实验大鼠骨质疏松的影响.方法 6月龄Wister雌性大鼠40只,按数字表法随机分为5组：假手术组、去卵巢组、雌激素组、高压氧组、高气压组,每组8只.除假手术组外,其余各组麻醉后切除双侧卵巢.1周后,假手术组和去卵巢组不加任何干预,高压氧组每日高压氧（表压0.1 MPa）暴露1次,每10次休息1周,共暴露12周;高气压组以压缩空气为加压介质在高压空气中暴露,其他条件和方法同高压氧组;雌激素组给予苯甲酸雌二醇腹腔注射,每周1次,共12周.实验结束后检测各组大鼠腰椎及股骨的骨密度、骨生物力学、骨形态计量学等指标.结果 去卵巢后,大鼠腰椎骨密度为（0.087 8 ±0.007 4）g/cm^2,股骨密度为（0.114 1 ±0.006 7）g/cm^2,腰椎骨最大应力为（216.32±45.26）N,股骨最大应力为（87.50 ±4.01）N,均较假手术组[分别为（0.123 0 ±0.087 8）g/cm^2、（0.129 0 ±0.034 0）g/cm^2、（381.58±28.75）N、（120.01±16.26）N]明显降低,差异有统计学意义（P＜0.05）;高压氧组以上各指标下降均不明显,与假手术组比较差异无统计学意义（P＞0.05）,与高气压组比较差异有统计学意义（P＜0.05）.结论 高压氧可以减轻去卵巢大鼠的骨密度和骨生物力学特性的降低,对绝经后骨质疏松的发生有一定的预防作用.

Objective To observe effect of hyperbaric oxygen （HBO） intervention on osteoporosis in rats with experimental premature ovary failureMethods Forty female Wister rats （with an age of 6 weeks） were randomly divided into 5 groups：the sham group,the deovary group,the estrin group,the HBO group and the hyperbaric air group,each consisting of 8 animals.With the exception of the sham group,all the other groups had ovariotomy bilaterally following anesthesia.One week later,the animals in the sham group and the deovary group were left there without any treatment,while the animals in the HBO group were exposed to HBO at a pressure of 0.1 MPa,one session a day for a succession of 10 days,then had one week interval every 10 sessions,with a total exposure time of 12 weeks.The animals in the hyperbaric air group were exposed to hyperbaric air at the same pressure and with the same treatment time and conditions.The animals in the estrin group received abdominal injection of estradiol benzoate once a week,for a succession of 12 weeks.After termination of the experiment,the animals of all the groups received lumbar vertebra and femora mineral density,bonc biomechanics and bone morphometry detection.Results After ovariotomy,lumbar vertebra mineral density was （0.087 8 ± 0.007 4） g/cm^2 and femora mineral density was （0.114 1 ± 0.006 7） g/cm^2.Lumbar vertebra biomechanics （216.32 ± 45.26） N and femora biomechanics （87.50 ± 4.01） N were significantly decreased,and statistical significance could be seen,as compared with those of the sham group [（381.58 ±28.75） N and（120 ± 16.26） N] （P 〈 0.05）.However,the levels of the above indicators did not reduce considerably and there was no significant difference,as compared with those of the sham group （P 〉 0.05）,but there was obviously significant difference in them,when it was compared with that of the hyperbaric air group （P 〉 0.05）.Conclusions HBO could delay the loss of bone mineral density and the reduced property of bone biomechanics in ovarectomized rats,and it had certain protective effect on the occurrence of osteoporosis after menopause.