摘要
目的探讨反复喘息婴幼儿外周血髓源性抑制细胞(MDSC)及Th17细胞比例及临床意义。方法随机选取急性发作期的反复喘息婴幼儿30例作为喘息组,同时选取同年龄段20例支气管肺炎患儿作为肺炎组,另选取同年龄段在该院外科住院的疝气、肾结石等非感染性、非肿瘤性疾病术前患儿23例作为对照组。流式细胞术检测各组患儿外周血中MDSC、Th17细胞的比例,采用Spearman相关性分析MDSC及Th17细胞比例的相关性。结果喘息组患儿MDSC占有核细胞的百分比明显高于肺炎组和对照组,肺炎组高于对照组(P<0.05)。喘息组Th17细胞占单个核细胞的百分比明显高于肺炎组及对照组,肺炎组与对照组Th17比较差异无统计学意义。喘息组外周血MDSC及Th17细胞比例呈正相关关系(r=0.645,P<0.01)。结论 MDSC及Th17细胞可能参与了婴幼儿反复喘息的发生发展。
Objective To determine the frequencies and significance of myeloid-derived suppressor cells(MDSCs) and T-helper 17(Th17) cells in peripheral blood of young children with recurrent wheezing. Methods Thirty young children with an acute exacerbation of recurrent wheezing were randomly enrolled. Twenty age-matched children with bronchopneumonia(pneumonia group) and 23 age-matched preoperative children with non-infectious or nonneoplastic diseases(hernia or renal calculus)(control group) were selected. The frequencies of MDSCs and Th17 cells in the peripheral blood were measured using flow cytometry and their correlation was determined by the Spearman's correlation coefficient. Results The percentage of MDSCs in nucleated cells was significantly higher in the wheezing group than in the pneumonia and control groups(P〈0.05), and it was significantly higher in the pneumonia group than in the control group(P〈0.05). The percentage of Th17 cells in mononuclear cells was significantly higher in the wheezing group than in the pneumonia and control groups(P〈0.05), but it showed no significant difference between the pneumonia and control groups(P〉0.05). The frequency of MDSCs was positively correlated with the frequency of Th17 cells in the wheezing group(r=0.645, P〈0.01). Conclusions MDSCs and Th17 cells may contribute to the pathogenesis of recurrent wheezing in young children.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2015年第4期312-316,共5页
Chinese Journal of Contemporary Pediatrics
基金
江苏镇江市第五批科技计划(科技发展支撑-社会发展
编号:SH2012033)
关键词
喘息
髓源性抑制细胞
TH17细胞
婴幼儿
Wheezing
Myeloid-derived suppressor cell
T-helper17 cell
Young child