诱发性肥胖对未成年SD大鼠糖脂类代谢的影响

Effect of Induced Obesity on Metabolism of Lipid and Glucose in Young SD Rats

目的探讨高脂饲料诱导肥胖对未成年SD大鼠糖脂代谢的影响。方法将30只21日龄雄性SD大鼠分为2组，分别给予基础饲料和高脂饲料，喂养至60日龄后进行胰岛素耐量实验，准确测定身长、体质量，采集血液检测血脂、血糖等生化指标；处死两组大鼠后，称量体脂含量，留取大鼠肝脏样本切片并HE染色作病理学观察。结果高脂饲料成功诱导未成年大鼠肥胖；肥胖大鼠血清甘油三酯（TG）、总胆固醇（TC）与正常组相比差异有统计学意义（尸〈0．05），而丙氨酸氨基转移酶（ALT）、血糖（FBG）、高密度脂蛋白（HDL-C）、胰岛素（Ins）等指标水平与正常组相比没有统计学差异（P〉0．05）；胰岛素耐量实验在各时间点两组间未见统计学差异；镜下观察肥胖大鼠肝细胞内包含大小不等的脂肪空泡。结论相较正常未成年大鼠，未成年肥胖大鼠脂质（TG、TC）代谢紊乱，FBG、ALT等有升高的趋势，HDL—C、Ins等则有下降趋势，同时，肥胖导致未成年大鼠形成脂肪肝。

Objective To study the effect of childhood obesity induced by high fat diet on the metabolism of glucose and lipid. Methods Thirty male SD rats （21-day-old） were randomly divided into 2 groups, and were given basic diet or high fat diet, respectively. At the 60 days old, the insulin tolerance test of rats of rats was proceeded, the body length and weight the rats were measured accurately, and the blood was collected for the measurement of biochemical markers. After the rats were sacrificed, the livers of rats were collected, sliced and stained for pathological observation. Result The obesity rat model induced by high fat diet was established successfully. The serum level of triglyceride （TG） and and total cholesterol （TC） in obese rats were higher than those of non-obese rats （P〈0.05）, but there were no differences in alanine aminotransferase （ALT）, fasting blood glucose （FBG）, high-density lipoprotein cholesterol （HDL-C）, and insulin （Ins） between groups （P〉0.05）. At each time point, there was no difference on the insulin tolerance test. Under the microscope, there were numbers of fat bubbles in liver cells of young obese rats. Conclusion Compared with the non-obese young rats, the metabolism of lipids （TG, TC） of the obese young rats was disordered, the level of FBG and ALT showed an increasing trend, the level of HDL-C and Ins showed a decreasing trend. Obesity induced by high fat diet can lead to fatty liver of young rats.