大鼠、小鼠、豚鼠和兔螺杆菌携带情况调查

Investigation on Helicobacter spp. Infection in Rat, Mouse, Guinea Pig and Rabbit

目的了解上海及周边地区大鼠、小鼠、豚鼠和兔螺杆菌携带情况。方法采集大鼠、小鼠、豚鼠回盲部内容物和兔粪便，抽提细菌基因组DNA，采用单重PCR和多重PCR方法进行螺杆菌检测。结果上述动物螺杆菌阳性率分别为50．5％（105／208）、37．9％（204／538）、36．7％（36／98）和4．9％（12／247）。Helicobacter．hepaticus、Hbil＆、H．rodentium三重PCR结果显示，实验用兔检测到H．hepaticus、H．bilis阳性。豚鼠阳性样本中检测到H．bilis、Hrodentium阳性。大鼠和小鼠样本中H．hepaticus、Hbil＆、H．rodentium均有阳性检出。结论上海及周边地区大鼠、小鼠、豚鼠和兔均存在不同程度的螺杆菌感染。

Objective To survey the Helicobacter spp. among rats, mice, guinea pigs and rabbits in Shanghai and surrounding area. Method The ileocecal region contents of rats, mice, guinea pigs and feces of rabbits were collected, and the bacterial genomic DNA were extracted. Both traditional PCR and multiplex PCR test were performed to investigate the Helicobacter spp, infection. Result The positive rate of Helicobacter spp. in rat, mouse, guinea pig and rabbit were 50.5%（105/208）, 37.9%（204/538）, 36.7% （36/98） and 4.9%（12/247） respectively. In addition, the triplex PCR test results of H.hepaticus, H.bilis, H. rodentium showed that H.hepaticus, H.bilis, infection can be detected in rabbit. H.bilis, H.rodentiurn can be detected in guinea pig. H.hepaticus, H.bilis, H.rodentium can be detected in rat and mouse. Conclusions There are different degrees of Helicobacter spp. infection in rats, mice, guinea pigs and rabbits of Shanghai and surrounding area.