稻瘟病抗性基因在主要育种亲本中的分布研究

Research on the Distribution of Rice Blast Resistance Genes in the Main Rice Breeding Parents

稻瘟病是由稻瘟病菌引起的具有广泛性和毁灭性的水稻病害。目前通过抗病亲本杂交聚合培育持久广谱的稻瘟病抗性品种是水稻稻瘟病防治最经济环保的途径,而利用分子标记辅助选择技术对水稻育种亲本抗性基因分布的研究是分子辅助聚合育种的基础。本研究以36个育种亲本为实验材料,进行苗瘟和穗颈瘟鉴定,结合特异性分子标记Pid3、Pita、Pikm、Pib、Piz,研究这些育种亲本的稻瘟病抗性基因的分布情况。同时,对恢复系材料蜀恢498的抗稻瘟病基因Pita和Pikm扩增测序并进行同源序列比对分析,初步在分子水平分析抗病基因与抗病表型的关系。

Rice blast is one of the most widespread and devastating diseases of rice which caused by fungal pathogen Magnaporthe grisea. The econmiec and friendly to control rice blast is to foster durable broad spectrum of hybrid polymeric blast by method of hybriding resistant parents. Molecular marker assisted selection technology is used to study the distribution of the resistance gene of rice breeding parent is the basis of molecular assisted breeding. In our research, 36 breeding parents as experimental materials for seedling blast and panicle blast identification, combined marker of Pid3, Pita, Pikm, Pib, Piz to study these rice blast resistance gene distribution on parental, while we cloned the blast resistance gene Pita, Pikm of the restorer line Shuhui498 and had the homologous sequence alignment analysis, preliminary analysis of the relationship between genes and disease resistance phenotype at the molecular level.