红掌两个bZIPs基因片段的克隆与低温响应特性分析

Molecular Cloning of Two bZIPs Genes and Their Response to Cold Stress in Anthurium

本研究以红掌‘Alabama’幼苗为材料,克隆了2个b ZIP转录因子基因Aab ZIP1和Aab ZIP2。Aab ZIP1片段长度462 bp,Aab ZIP2片段长度为369 bp,两基因核苷酸序列的相似度为46.97%,推定的氨基酸序列相似性为31.17%。Blastx发现两个基因均具有亮氨酸拉链的保守结构域,具备b ZIP转录因子家族的基本特征。RT-PCR分析检测表明,低温处理后,Aab ZIP1基因为组成型表达,不响应低温胁迫;Aab ZIP2基因表达受低温诱导,其表达强度随着低温处理时间的延长而增强,Aab ZIP2可能在红掌的低温胁迫响应过程中起重要作用。

In this study, Anthurium seedlings‘Alabama＇were used as experimental materials. Using PCR method,we cloned two b ZIP genes from Anthurium seedlings, named Aab ZIP1 and Aab ZIP2. The length of Aab ZIP1 gene fragment is 462 bp, and the length of Aab ZIP2 gene fragment is 369 bp. The nucleotide sequences of Aab ZIP1 and Aab ZIP2 showed 46.97% similarity, and the putative amino acid of the two genes showed 31.17% similarity. The results of blastx showed that the two genes both had basic region leucine zipper conserved domain, and the two genes had the basic characteristics of b ZIPs transcription factor family. RT-PCR analysis indicated that Aab ZIP1 was constitutively expressed, and it didn＇t in response to low temperature stress; Aab ZIP2 was induced by low temperature, and its intensity of expression was increased with the prolongation of low temperature treatment.These results indicated that Aab ZIP2 might play a key role in Anthurium cold stress response.