火焰兰茎段侧芽离体再生及褐化的研究

In Vitro Regeneration and Browning of Renanthera coccinea Loureiro Stem Buds

以火焰兰的茎段为外植体,探讨不同植物激素配比对茎段侧芽萌发和生根壮苗的影响,以及不同培养基配方和不同添加剂对火焰兰生长过程中褐化现象的抑制作用。结果表明:最佳侧芽诱导培养基为MS+6-BA3.0 mg/L+NAA 0.5mg/L,培养40 d,后诱导率可达97.95%;添加活性炭0.3 g/L可有效抑制侧芽诱导过程中的褐化现象,褐化率仅为13.17%;1/2 MS+6-BA 0.2 mg/L+NAA 1.0 mg/L+AC 0.5 g/L生根效果最好,生根率达到100%,且植株生长健壮。利用火焰兰茎段侧芽能够离体再生获得瓶苗,建立高效的火焰兰茎段为外植体的离体再生体系,为扩大拓宽火焰兰组织培养外植体来源和快速繁殖技术提供技术支撑。

The present study establishing efficient and stable Renanthera in vitro regeneration system by using stem buds, aim to expand the Renanthera explant sources and provide the technical support of rapid propagation technique of Renanthera. This research by using stem buds of Renanthera as explants, discusses the effect of different plant growth regulator combinations on the stem bud＆#39;s inducing and rooting culture, and inhibitory effect of different medium and different additives on the growth process of Renanthera imschootiana Browning. Result： The optimal culture medium for stem bud inducement was MS＋6-BA 3.0 mg/L＋NAA 0.5mg/L, with the inductivity rate was 87.5% after 40 days of induction;adding activated carbon can control browning phenomenon of stem bud inducing culture effectively, with the browning rate was 13.17%; the optimal culture medium for rooting was 1/2MS＋6-BA 0.2 mg/L＋NAA 1.0mg/L＋AC 0.5 g/L, with the rooting rate was 100%. Using the stem buds of Renanthera can in vitro regenerate plantlets, it＆#39;s lay the foundation for the rapid propagation of Renanthera.