滋肾疏肝胶囊对焦虑模型大鼠海马区5-HT1AR表达的影响

Effect of Zishen Shugan Capsule on 5-HT1AR Expression in Hippocampus of Anxiety Rat Model

目的 :通过建立焦虑大鼠模型,研究中药复方滋肾疏肝胶囊的抗焦虑作用及其机制。方法 :采用不确定性空瓶刺激法制备焦虑大鼠模型,24只大鼠随机分为4组,即空白对照组、模型组、地西泮片给药组、滋肾疏肝胶囊给药组,除空白对照组外,分别以蒸馏水、地西泮片、滋肾疏肝胶囊灌胃给药。运用RT-PCR和Western Blotting方法分别检测5-羟色胺1A受体(5-HT1AR)m RNA及蛋白表达水平,单因素方差分析相关数据。结果:与空白对照组比较,模型组大鼠攻击行为和修饰行为频率均显著性升高(P<0.01),高架十字迷宫实验OE%和OT%值均显著性下降(P<0.01),海马区5-HT1AR m RNA及蛋白表达均显著性降低(P<0.01)。与模型组比较,滋肾疏肝胶囊给药组大鼠攻击行为和修饰行为频率均显著性降低(P<0.01),OE%和OT%值均显著性升高(P<0.01),海马区5-HT1AR m RNA及蛋白表达均显著性升高(P<0.01)。结论:滋肾疏肝胶囊对焦虑症大鼠具有明显的治疗效果,5-HT1AR基因可能是该药物的作用靶点。

Objective：To study the antianxiety effect and its mechanism of Zishen Shugan capsule by establishing anxiety rat model. Methods：The anxiety rat model was established by uncertainty stimulation of empty bottle. The 24 rats were randomly divicled into 4 groups：control group,model group,diazepam tablet group and Zishen Shugan capsule group. Except the control group,other groups were given distilled water,diazepam tablet and Zishen Shugan capsule by gavage respectively. The expressions of m RNA and protein of 5-hydroxy tryptamine 1A receptor（5-HT1AR） were respectively detected by RT-PCR and Western blotting.The data was analyzed by One-factor analysis of variance. Results：Compared with control group,the frequency of both aggressive behavior and grooming behavior of model group increased significantly（P 〈0.01）,the open arm entry percent（OE%） and the open arm time percent（OT%） of elevated plus-maze test de creased significantly（P 〈0.01）,the m RNA and protein expressions of 5-HT1 AR in the hippocampus decreased significantly（P 〈0.01）. Compared with model group,the frequency of both aggressive behavior and grooming behavior of Zishen Shugan capsule group decreased significantly（P 〈0.01）,the OE% and OT% increased significantly（P 〈0.01）,the m RNA and protein expressions of 5-HT1 AR increased significantly（P 〈0.01）. Conclu sion：Zishen Shugan capsule had significant curative effect on anxiety rat and the gene 5-HT1 AR could be the drug target.