不同频率电针及艾灸对心律失常家兔的影响

Effects of Different-frequency-electroacupuncture and Moxibustion Stimulation of “Neiguan”(PC 6)on Arrhythmia in Rabbits

目的:探讨电针频率及艾灸对家兔心律失常的影响。方法:将100只家兔随机分为模型组、3 Hz电针组、20Hz电针组、100Hz电针组、艾灸组,每组20只。通过静脉注射25μg/kg的乌头碱诱发家兔心律失常。电针组分别予3Hz、20Hz、100Hz 3种频率电针刺激"内关"穴,艾灸组予艾条灸"内关"穴。用生物信号处理系统记录心电,以心律失常开始时间、持续时间为观察指标,并用酶联免疫吸附法检测血清高敏C反应蛋白(hs-CRP)浓度变化。结果:5组间心律失常开始时间差异无统计学意义(P>0.05);20 Hz、100Hz这2种频率的电针能缩短乌头碱诱发的家兔心律失常持续时间,与模型组比较差异有统计学意义(P<0.01,P<0.05),20Hz电针组与3Hz电针组间比较差异亦有统计学意义(P<0.05);静脉注射乌头碱60min后5组家兔hs-CRP值均较给药前明显升高(P<0.001),而20 Hz电针组给药后60min hs-CRP浓度较模型组相应时段明显降低(P<0.05)。结论:中、高两种频率的电针均能对抗乌头碱诱发的家兔心律失常,以20Hz的电针效果为佳。

Objective To observe the effect of different-frequency-electroacupuncture （EA） and moxibustion stimulation on rabbits＇ arrhythmias. Methods Newzealand rabbits were randomly divided into arrhythmia model （n = 18）, EA-3 Hz （n = 19）, EA-20 Hz （n = 19）, EA-100 Hz （n = 18） and moxibustion （n = 18） groups. The arrhythmia model was induced by intravenous injection of 25 lag/kg aconitine. EA （3 Hz, 20 Hz or 100 Hz, 3.5-4 V） or moxibustion was applied to bilateral ＂Neiguan＂ （PC 6） for 20 min and electrocardiogram （ECG） of the standard limb lead ]] was recorded by using a Biosignal Processing Instru- ment. The concentration of serum hypersensitive C reactive protein （hs-CRP） content was detected by using ELISA method. Results Following EA intervention, the duration of aconitine-induced arrhythmia was significantly shorter in both EA-20 Hz and EA-100 Hz groups than in the model group （P〈0.05, P〈0.01）, and the serum hs-ORP level in the EA-20 Hz group was significantly lower than that of the model group （P^0.05）. No significant changes were found in the duration of arrhythmia in both EA- 3 Hz and moxibustion groups, and in the serum hs-CRP level in the EA-3 Hz, EA-100 Hz and the moxibustion groups （P〉0.05）. Conclusion EA stimulation of PC 6 at 20 Hz and 100 Hz has an anti-arrhythmic effect in aconitine-induced arrhythmia rabbits.