HPLC法同时测定三七花盘中人参皂苷Rb1和人参皂苷Rb3的含量

Content determination of ginsenoside Rb1 and ginsenoside Rb3 in flower disc of Panax notoginseng by HPLC

目的：建立HPLC法同时测定三七花盘中人参皂苷Rb1和人参皂苷Rb3的含量。方法采用ACE 5 C18（250mm×4.6mm，5μm）色谱柱；流动相：乙腈-0.05%磷酸溶液（32∶68）；流速：1.0mL/min；检测波长：203nm；柱温：25℃。结果人参皂苷Rb1和人参皂苷Rb3分别在10.25～143.50mg/L（r=0.9993， n=7）和10.94～153.16mg/L（r=0.9997，n=7）范围内呈良好线性关系；平均回收率（n=6）分别为98.78%和98.17%。结论本测定方法简便、快速、准确，为三七花盘的综合开发利用提供了参考，有利于控制三七花盘的质量。

Objective To establish a high performance liquid chromatographic（HPLC）method for simultaneous detection of ginsenoside Rb1 and ginsenoside Rb3 in flower disc of Panax notoginseng.Methods ACE5 C18 column （250mm×4.6mm,5μm）was used with a mixture of acetonitrile-0.05% phosphoric acid（32∶68,V：V）as the mobile phase.The flow rate was 1.0mL/min,the detection wavelength of 203nm,the column temperature of 25℃.Results The linear range of ginsenoside Rb1 and ginsenoside Rb3 were 10.25~143.50mg/L（r=0.9993,n=7）and 10.94~153.16 mg/L （r=0.9997,n=7）respectively.The average recovery rates were 98.78% and 98.17%,respectively.ConclusionThe method is simple,accurate and sensitive with good reproducibility.It can provide references for resources development and be used for quality controlling of flower disc of Panax notoginseng.