HPLC法测定曲安奈德新霉素贴膏中醋酸曲安奈德的含量

Determination of triamcinolone acetonide acetate in triamcinolone acetonide and neomycin paste by HPLC

目的：建立曲安奈德新霉素贴膏中醋酸曲安奈德含量的HPLC测定方法。方法采用反相高效液相色谱法，使用DIONEX Acclaim 120 C18（250mm×4.6mm，5μm）色谱柱，以甲醇-水（60∶40）作为流动相，检测波长240nm，流速1.0mL/min，柱温为30℃。结果醋酸曲安奈德在0.05～0.50μg范围内线性较好（r=0.9999）,平均回收率为98.09%（RSD为0.73%）。结论该方法快速、简便、可靠、准确度高、重复性好，处方中其他成分对测定亦无干扰，分离效果好，可用于曲安奈德新霉素贴膏中醋酸曲安奈德含量测定。

ObjectiveTo establish a method for content determination of triamcinolone acetonide acetate in triamcinolone acetonide and neomycin paste by HPLC.Methods The experimental condition of the RP-HPLC method was as follows：DIONEX Acclaim 120 C18（250mm×4.6mm,5μm）,with linear gradient elution using methanol and water （60∶40）,The detected wavelength was 240 nm,the flow rate was 1.0 ml/min,The column temperature was at 30℃. ResultsThe fluocinolone acetonide curve was linear in the range of 0.05~0.50μg（r=0.9999）.The average recovery rate was 98.09% withRSD of 0.73%.ConclusionThe method is simple,reliable,accurate and It could be used in the determination of triamcinolone acetonide acetate in triamcinolone acetonide and neomyc in paste.