HPLC法测定磷酸奥司他韦胶囊的含量

Content determination of oseltamivir phosphate capsules by HPLC

目的：建立一种测定磷酸奥司他韦胶囊含量的方法。方法采用高线液相色谱法测定磷酸奥司他韦的含量，色谱柱：Kromasil C8（250mm×4.6mm，5μm）,流动相为0.05mol/L磷酸二氢钾溶液（40%氢氧化钾调节pH至5.5）-甲醇-乙腈（70：20：10），检测波长为207nm，柱温30℃，流速1.0mL/min，进样量20μL。结果磷酸奥司他韦在1～50μg范围内线性关系良好（r=0.9992）。平均回收率为99.8%,RSD=0.9%（n=9）,稳定性、精密度、重复性试验的相对标准偏差均小于0.8%。结论此方法操作简便快捷、准确度高,适用于磷酸奥司他韦胶囊的含量测定。

ObjectiveTo establish an method for the determination of Oseltamivir Phosphate capsules.Methods HPLC method was adopted.The separation was performed on Kromasil C8（250mm×4.6mm,5μm）, column with mobile phase consisted of 0.05mol/L postassiam dihydrogen phosphate （adjust pH to 5.5 with 40% potassium hydroxide）- methanol- acetonitrile（70：20：10）. UV detection wavelength was set at 207nm,the column temperature was maintained at 30℃,the flow rate was 1.0mL/min and the sample size was 20μL.Results The line arrange of Oseltamivir Phosphate was 1-50μg,the correlation coefficient was 0.9990 and the average recovery was 99.8%（RSD=0.9%,n=9）.RSDs of stability test,precision test,reproducibility test were all less than 0.8%. Conclusion The method is simple,quick, accurate and suitable for the content determination of Oseltamivir Phosphate capsules.