补肾活血中药对老年小鼠膝关节炎的影响

The Kidney-invigorating and Blood-activating Chinese Medicine on the Knee Arthritis of Elderly Mice

目的根据中医肾主骨的思想,研究补肾活血中药对老年小鼠膝骨关节炎(OA)的影响。方法昆明种雄性小鼠,按鼠龄分成老年组、壮年组、中年组和药物组,每组各10只,药物组在正常饮食基础上加食补肾活血中药,余3组正常饮食,3周后采用实时荧光定量PCR和蛋白免疫印迹技术检测不同年龄组小鼠膝关节软基质金属蛋白酶1(MMP-1),1型胶原蛋白(COL1)和2型胶原蛋白(COL2)的mRNA和蛋白水平的表达。结果 MMP-1和COL1在老年组、药物组中表达增高,药物组增高程度低于老年组,而COL2在老年组、药物组中表达降低,药物组降低程度低于老年组(P<0.05)。结论随着小鼠年龄的增加MMP-1和COL1的表达增加,COL2表达下调,补肾活血中药能够一定程度减少OA的发生。

Objective To expl ore the relationship between age and knee osteoarthritis and find a method to prevent it following the theory of kidney facilitating bone in the Chinese traditional medicine.Methods The elderly mice were fed with kidney-invigorating and blood-activating Chi-nese mediic ne.The quantitative PCR and protein immunoblot technology were used to detect the expression of genes and proteins level of matrix metalloproteinase 1 （ MMP-1 ） , collagen type 1 gene （COL1） and type 2 collagen （COL2）in different age group and medicine grou p. Results Both in mRNA and protein level,MMP -1a nd COL1 expression increased in the elderly group and declined after them ice fed with traditional Ch inese medi cine, while the expressions of COL2 in the medicine group were higher than those of the elderly group （ P〈0.05） .Conclu-sion With the increa se of the age in mice, the expres ions of MMP-1 and COL1 increased and the COL2 declined, the kidney-invigo rating and blood-activating Chinese medicine could re-verse this proces effectively, and reduce the occurrence of osteoarthritis.