健骨伸筋汤对大鼠膝关节软骨细胞增殖与凋亡的影响

Effects of Jiangu Shenjin decoction on cell proliferation and apoptosis of rat's knee joint chondrocyte

目的探讨健骨伸筋汤对大鼠膝关节软骨细胞增殖与凋亡的影响。方法采用改良的Hulth法造模,造模2周后,开始对空白对照组、模型组生理盐水灌胃;对照组壮骨关节丸灌胃;治疗组分低、高浓度进行中药灌胃,第13周处死,通过免疫组化、TUNEL法检测软骨细胞的增殖与凋亡。说明健骨伸筋汤可以通过调节软骨细胞的增殖与凋亡防治骨关节炎。结果健骨伸筋汤方含药血清各浓度组的软骨细胞增殖指数值明显高于对照组及模型组(P<0.05),软骨细胞凋亡率明显低于对照组及模型组(P<0.05)。结论健骨伸筋汤可以促进软骨细胞的增殖,还可以抑制软骨细胞异常凋亡,从而起到防治骨关节炎的作用。

Objective To explore the effects of Jiangu Shenjin decoction on the cell proliferation and apoptosis of rat＇s chondrocyte. Methods By using improved Hulth method molding,after building 2 weeks,the blank control group and model group applying normal saline lavage; The control group using strong bone joints pill to lavage; Treatment group using low and high concentration of Chinese traditional medicine to lavage respectively. On the 13 weeks executed,through immunohistochemistry and TUNEL method to detect cartilage cell proliferation and apoptosis. Explain Jiangu Shenjin decoction can prevented and treatment of osteoarthritis by adjust the cartilage cell proliferation and apoptosis.Results The increased index of all dosage of Jiangu Shenjin decoction were significantly higher than control group and molding group（ P〈 0. 05）,and the apoptosis rate of chondrocyte was significantly lower than control group and molding group（ P〈 0. 05）. Conclusion Jiangu Shenjin decoction can improve the cell proliferation activity and obstruct the abnormal apoptosis of chondrocyte,so as to prevent osteoarthritis from happening.