云南重楼内生细菌的分离鉴定及系统发育树分析

Isolation and Identification of Endophytic Bacteria in Paris polyphylla Smith var.yunnanensis(Franch) and Phylogenetic Analysis

利用云南文山采集的人工栽培和野生云南重楼Paris polyphylla Smith var.yunnanensis(Franch)根茎,对其内生细菌进行了分离、纯化及鉴定。分离得到40和44株云南重楼内生细菌。经16S rDNA测序,在NCBI中比对,MEGA4建系统进化树,所得内生细菌分别鉴定为分属于6和8个属。其中,人工栽培云南重楼内生细菌中,芽孢杆菌属(Bacillus)和肠杆菌属(Enterobacter)分离频率分别为35%、27.5%,是其优势内生细菌群;野生云南重楼内生细菌中,芽孢杆菌属(Bacillus)和克雷伯氏菌属(Klebsiella)分离频率分别为36.4%、20.4%,是其优势内生细菌群。本研究结果体现了人工栽培和野生云南重楼内生细菌分布的差异及云南重楼根中内生细菌的多样性,也为后续重楼内生菌的多样性开发提供了理论和实验基础。

In this manuscript,the endophyte of Paris polyphylla Smith var.yunnanensis（Franch）was isolated and identified.40 and 44 endophytic bacteria were isolated from the root of cultivated or wild Paris polyphylla Smith var.yunnanensis in Wenshan,Yunnan province.They were then identified and categorized as 6 and 8 genera by 16 S rRNA gene sequenceing.Bacillus and Enterobacter were dominant genera in cultivated of Paris polyphylla Smith var.yunnanensis because they accounted for 35% and 27.5% respectively in the identified bacteria;Bacillus and Klebsiella were dominant genera in wild Paris polyphylla Smith var.yunnanensis and accounted for 36.4% and 20.4% respectively.The results reflected the difference and biodiversity of endophytic bacteria between cultivated Paris polyphylla Smith var.yunnanensis and wild ones.This study provides theoretical and experimental basis for subsequent development of Paris polyphylla Smith var.yunnanensis.