注射用核糖核酸Ⅱ的抗肿瘤活性测定

Determination of Anti-carcinoma Activity of Ribonucleic Acid Ⅱ

将不同浓度梯度核糖核酸Ⅱ作用于7种细胞系,采用WST-8比色法测定其对细胞增殖的抑制情况,选择较敏感的人红白血病细胞K562作为试验对象,考察了细胞接种密度、药物作用时间等条件的影响,初步建立了注射用核糖核酸Ⅱ体外抗肿瘤活性的测定方法。核糖核酸Ⅱ在0.02~100 mg/ml范围内线性关系良好。采用本法测定了10批样品的抗肿瘤活性,结果对K562细胞的抑制率均大于55%。

Seven cell lines were treated with different concentrations of RNA Ⅱ. The cell proliferation was determined by WST-8 colorimetric assay, and the sensitive cell line, human leukemia cell line K562, was chosen as the experiment object. Experiment conditions including cell density and incubation time were investigated. It was linear for RNA Ⅱ in the range of 0.02- 100 mg/ml. The anti-carcinoma activities of 10 batches of samples were determined by this method, and their inhibition rates to K562 cells were all over 55%.