植酸酶对黄金鲫钙磷代谢的影响

Effects of phytase on calcium ami phosphorus metabolism in Carassium auratus

为研究植物性饲料中添加不同梯度的中性植酸酶对黄金鲫（Carassiusauratus）钙磷代谢的影响，以初始体质量为（10．71±0．16）g的黄金鲫幼鱼为研究对象，随机分为5组，对照组添加磷酸二氢钙，各试验组添加中性植酸酶，依次添加0、300、600、900、1200U／kg，每组有3个平行组，饲养60d。结果表明：当植酸酶水平为600U／kg时，黄金鲫肠道蛋白酶比活力提高了15．78％。钙、磷表观消化率提高了6．48％和14．17％，骨钙与骨磷分别提高了13．23％和4．93％．血钙提高了61．61％（P〈0．05）；当饲料植酸酶水平增加到900U／kg时，黄金鲫氨氮与磷酸盐排泄量分别降低了19．68％和13．47％（P〈0．05）。根据本试验研究结果，以钙磷代谢为指标，在黄金鲫全植物蛋白源饲料中的植酸酶适宜添加量为600～900U／kg。

The experiment was conducted to study the effects of phytase levels in diet on calcium and phosphorus metabolism of Carassius auratus.Carassius auratus with an average weight of （10.71 ±0.16）g were randomly divided into 6 treatments and 3 replicates per treatments,control group feed calcium dihydrogen phosphate,test group feed neutral phytase.The test group were supplemented with different levels （0,300,600,900,1200 U/kg） of phytase.The trial period was 60 days.The results showed when the level of phytase was 600 U/kg,the protease specific activity increased by 15.78%, apparent digestibility of calcium and phosphorous increased by 6.48% and 14.17% ,vertebrae calcium and phosphorous increased by 13.23% and 4.93% ,blood calcium increased by 61.60% ;and when the level of phytase was 900 U/kg the ammonia nitrogen and phosphorous salts reduced by 19.68% and 13.47%.According to the test results,based on calcium and phosphorus metabolism indexes, the appropriate additive scope of phytase was 600 - 900 U/kg.