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小花黄堇挥发油成分的GC-MS分析 被引量:2

GC-MS Analysis of Essential Oil Component from Corydalis racemosa
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摘要 目的:研究小花黄堇挥发油的化学组成成分。方法:以水蒸气蒸馏法提取小花黄堇挥发油,采用气相色谱-质谱(GC-MS)联用技术鉴定其化学成分。气相色谱条件为HP-5MS弹性石英毛细管柱;升温程序为初始柱温100℃,保持2 min,以10℃/min升至150℃,再以2℃/min升至180℃,最后再以10℃/min升至280℃,保持1 min;载气为高纯氦气;流量为1.0 ml/min;进样口温度为280℃;进样量为1.0μl。质谱条件为EI离子源,电子能量70 e V,离子源温度为230℃,四极杆温度为150℃,溶剂延长3 min,扫描范围35-550 amu。用离子流色谱峰面积归一法计算各化学成分的相对含量。结果:从小花黄堇挥发油中共分离出55个色谱峰,鉴定了44个化合物,占总量的89.4%;含量在1%以上的有19个,以棕榈酸的含量最高(29.53%),其次为叶绿醇(7.71%)、亚麻酸甲酯(6.55%)和六氢法呢基丙酮(6.14%)等。结论:该文首次采用GC-MS联用技术研究小花黄堇挥发油的化学成分,主要有脂肪酸、酮、烷烃、醇、酯、醛类等化合物,以棕榈酸的含量最高,可为小花黄堇的综合利用提供科学依据。 OBJECTIVE:To study the chemical compositions of the essential oil from Corydalis racemosa. METHODS:Steam distillation method was used to extract the essential oil from C. racemosa and GC-MS was used to detect the chemical compositions. GC condition was as follows as HP-5MS elastic quartz capillary column;the oven temperature program started at 100 ℃ for2 min and increased at a rate of 10 ℃/min up to 150 ℃,then at a rate of 2 ℃/min up to 180 ℃,finally increased again at a rate of 10 ℃/min up to 280 ℃ for 1 min;the flow rate of the carrier gas(helium)was 1.0 ml/min;the temperature was 280 ℃ and volume was 10 μl. MS condition was as followsas EI iron source,electron energy of 70 e V,ion source temperature was 230 ℃,quadrupole temperature was 150 ℃,solvent delay time was 3 min and mass scan range was 35-550 amu. Ion chromatogram peak area normalization method was used to calculate the relative content of each chemical composition. RESULTS:55 chemical compositions were obtained from essential oil from C. racemosa and 44 of them were identified(89.4%). There were 19 compounds,of which relative contents were more than 1%. Among these,palmitic acid showed the highest content(29.53%),followed by phytol(7.71%),methyl linolenate(6.55%)and hexahydrofarnesyl acetone(6.14%),etc. CONCLUSIONS:The essential oil of C. racemosa is studied by GC-MS for the first time,they are mainly fatty acid,ketone,alkane,alcohol,ester,aldehydes and palmitic acid,etc.;palmitic acid shows the highest content. It provides scientific evidence for the comprehensive utilization of C. racemosa.
出处 《中国药房》 CAS 北大核心 2015年第12期1686-1688,共3页 China Pharmacy
基金 福建中医药大学科技项目(No.X2012011)
关键词 小花黄堇 水蒸气蒸馏 挥发油 气相色谱-质谱联用技术 成分分析 Corydalis racemosa Steam distillation Essential oil GC-MS Component analysis
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