HPV-DNA分型检测在尖锐湿疣检测诊断中的应用研究

Application of HPV- DNA genotyping in the detection and diagnosis of genital warts

目的:对HPV-DNA分型检测在尖锐湿疣检测诊断中的应用展开研究分析。方法:随机选取我院收治的200例尖锐湿疣患者及高危人群,采用荧光定量聚合酶链反应对所选患者进行HPV-DNA分型检测,总结分布特征。结果:尖锐湿疣患者61例,HPV-DNA分型检测结果单型感染中,6/11型为90.0%,16/18型为3.3%,6/11型、16/18型混合感染为3.3%。有尖锐湿疣病史患者33例,HPV-DNA检出率为36.4%;配偶或性伴侣为尖锐湿疣患者25例,HPV-DNA检出率为28.0%;非尖锐湿疣患者35例,HPV-DNA检出率为20.0%;不洁性行为史自检患者46例,HPV-DNA检出率为26.1%。结论:尖锐湿疣患者及高危人群HPV-DNA分型检测中HPV以6/11型最为多见,尖锐湿疣高危人群HPV-DNA阳性率均较高,且女性HPV-DNA阳性率高于男性,临床应加强对此类高危人群的HPV-DNA分型检测。

Objectives： To research and analyze the HPV- DNA genotyping in detection and diagnosis of genital warts. Methods： 200 genital warts patients and high- risk patients who received treatment in our hospital were randomly selected. Fluorescence quantitative polymerase chain reaction was used to do the HPV- DNA genotyping test for the selected patients. Genotyping distribution was summed. Results： There were 61 patients with genital warts. In the HPV- DNA genotyping results of single type of infection,6 /11 type,16 /18 type and 6 /11 and16 / 18 mixed infection accounted for 90. 0%,3. 3% and 3. 3%,respectively. There were 33 patients with a history of genital warts. HPV- DNA detection rate was 36. 4%; there were 25 patients＇ spouses or sexual partners with genital warts. HPV- DNA detection rate was 28. 0%; there were 35 patients with non- genital warts. HPV- DNA detection the rate was 20. 0%; there were 46 patients with history of dirty sex self- test. HPV- DNA detection rate was 26. 1%. Conclusions： Among genital warts patients and high- risk patients,6 /11 type is the most common type in HPV genotyping test. In populations with high- risk genital warts,HPV- DNA positive rate is high and the HPV- DNA – positive rate of women is higher than men＇s. In clinic,HPV- DNA genotyping test should be strengthened for the high- risk group.