摘要
Objective To investigate effects of clenbuterol （CLB） on testicular ultrastructure of rat. Methods Twenty adult male Sprague-Dawley rats were randomly divided into four groups （5 rats per group）. CLB solved in normal saline solution was given at the dose of O mg/kg body weight （bw） （group A, as control）, 0.4 mg/kg bw （group B）, 2.0 mg/kg bw （group C）, and 18.5 mg/kg bw （group D）for 14 d by garage consecutively, respectively. Transmission electron microscopy was used to observe changes on testicular ultrastructure. Results In group B, some small vacuoles were found in Sertoli cells. In groups C and D, vacuoles were common in Sertoli cells and spermatogonia. The phenomenon of vacuolation in group D was more severe than that in group C. In group D, basal membrane showed some irregular and wrinkled changes, Leydig cells had more vacuoles and increased lipid droplets. Conclusion Testicular ultrastructure of rat had pathological changes after CLB exposure, and the alterations became more severe with the increasing doses.
Objective To investigate effects of clenbuterol （CLB） on testicular ultrastructure of rat. Methods Twenty adult male Sprague-Dawley rats were randomly divided into four groups （5 rats per group）. CLB solved in normal saline solution was given at the dose of O mg/kg body weight （bw） （group A, as control）, 0.4 mg/kg bw （group B）, 2.0 mg/kg bw （group C）, and 18.5 mg/kg bw （group D）for 14 d by garage consecutively, respectively. Transmission electron microscopy was used to observe changes on testicular ultrastructure. Results In group B, some small vacuoles were found in Sertoli cells. In groups C and D, vacuoles were common in Sertoli cells and spermatogonia. The phenomenon of vacuolation in group D was more severe than that in group C. In group D, basal membrane showed some irregular and wrinkled changes, Leydig cells had more vacuoles and increased lipid droplets. Conclusion Testicular ultrastructure of rat had pathological changes after CLB exposure, and the alterations became more severe with the increasing doses.
基金
supported by Science and Technology Planning Project of Guangzhou City,China(No.2013 00000114)
