优化治疗神经胶质瘤复方抗瘤丸的实验研究

Study on Optimization of KLW for Treating Glioma

目的:通过实验研究,优化治疗神经胶质瘤中药复方抗瘤丸(KLW)的组成,得出最佳处方配伍。方法:采用大鼠神经胶质瘤C6细胞BALB/c裸鼠移植瘤模型,将模型裸鼠按瘤块体积随机分为10组(模型组,阳性药对照组(替莫唑胺TMZ组),KLW-1、KLW-2、KLW-3、KLW-4高、低剂量组,每组7只裸鼠。)连续给药15 d,检测各组裸鼠体重、瘤块体积、瘤块重量、微血管密度(MVD)、血管内皮生长因子(VEGF)、凋亡细胞、Bcl-2,Bax蛋白表达水平。结果:与模型组比较,KLW-1高、低剂量组,KLW-2、KLW-4高剂量组,TMZ组瘤体积显著减小(P<0.05);KLW-4高剂量组,TMZ组移植瘤的MVD值明显降低(P<0.05);KLW-2、KLW-4高剂量组,TMZ组移植瘤VEGF灰度值明显增高(P<0.05);KLW-1、KLW-2、KLW-4高剂量组,TMZ组细胞凋亡率显著增高(P<0.05);各给药组Bcl-2/Bax的比例增加。结论:处方KLW-1高、低剂量组,KLW-2、KLW-4高剂量组对C6移植瘤的生长具有较好的抑制作用。

Objective： To optimize compound KLW,and screen for the best prescription. Methods： C6 cells in BALB / c nude mice transplantation tumor model was established,and then randomly divided into ten groups（ the model control group,the TMZ group,and low,high dose KLW-1,KLW-2,KLW-3,KLW-4 group,each group including seven rats）. The rats were orally administered with medication for 15 days. The study measured weight of mice,the transplanted tumor volume and weight,MVD,VEGF,apoptotic cells,the Bcl-2,Bax protein expression level. Results： Compared with model control group,low,high dose KLW-1,high dose KLW-2 and KLW-4 TMZ group of tumor volume significantly reduced（ P 0. 05）; high dose KLW-4,TMZ group of tumor MVD decreased（ P 0. 05）; high dose KLW-2 and KLW-4,TMZ group of tumor VEGF value increased（ P 0.05）; high dose KLW-1,KLW-2 and KLW-4,TMZ group of cell apoptosis rate increased（ P 0. 05）; each dose group the Bcl-2/ Bax ratio increases. Conclusion： Low and high dose of KLW-1and KLW-2,high dose KLW-4 have good effects on the growth of C6 transplantation tumor inhibition.