广粉1号粉蕉组培快繁技术研究

Study of the techniques of fast propagation and tissue culture for dwarf banana

对广粉1号粉蕉腋芽的消毒方法、外植体诱导、幼芽增殖、生根培养进行研究。结果表明,采用75%酒精60 s+0.1%升汞(第1次10 min+第2次10 min)的消毒方法,比较适于广粉1号粉蕉外植体消毒,易获得理想的无菌体系;在不定芽的诱导初期阶段,6-BA浓度采用5.0 mg/L,最适合广粉1号粉蕉外植体的诱导;在幼芽的中高代增殖培养阶段,适宜采用MS+6-BA 4.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L配方;在幼芽的高代增殖培养阶段,适宜采用MS+6-BA 3.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L配方;在幼芽的生根阶段,适宜采用MS+IBA2.5 mg/L+NAA 0.2 mg/L配方,幼苗生根快、根系壮实、发达,成活率高。

The sterilize method, induction of explant and multiplication of bud and rooting culture for dwarf banana bud were studied. The results indicated the sterile treatment of 75% alcohol for 60 seconds＋0.1% Hgcl2 （first 10 min ＋ second time 10min） is more appropriate in sterilize of explants for dwarf banana, it was easy to obtain ideal aseptic system with the method. 6-BA 5.0 mg/L was suitable for the inductions in the early stages of bud induction. MS＋6- BA 4.0 mg/L＋AD 3.0 mg/L＋NAA 0.1 mg/L was suitable in the stage of middle and high proliferation culture for bud. MS＋6-BA 3.0 mg/L＋AD 3.0 mg/L＋NAA 0.1 mg/L was suitable in the stage of high proliferation culture for bud. MS＋IBA 2.5 mg/L＋NAA 0.2 mg/L was suitable in shoots rooting stage of bud. Seedling rooted quickly, and roots were stout with high survival rate.