兔脊髓缺血再灌注损伤后能量代谢与NF-κ Bp65表达变化

Changes of Energy Metabolism and the Expression of NF-κ Bp65 after Spinal Cord Ischemia-Reperfusion Injury in Rabbits

目的:探讨脊髓缺血再灌注损伤(SCIRI)中能量代谢与NF-κBp65的变化规律。方法:40只新西兰大白兔采用肾下腹主动脉阻断45 min,建立脊髓缺血再灌注损伤模型。分别于缺血前、缺血45 min及再灌注30、60、120 min时取脊髓测定腺苷酸(ADP、AMP、ATP)、活性氧(ROS)、核转录因子-κBp65(NF-κBp65)、抑制蛋白-κBα(I-κBα)和细胞间黏附分子-1(ICAM-1)蛋白表达变化。结果:与缺血前比较,缺血45 min及再灌注30、60、120 min时脊髓腺苷酸及I-κBα均降低(P<0.01),ROS、NF-κBp65和ICAM-1均升高(P<0.01)。结论:SCIRI中ICAM-1和NF-κBp65表达上调加重脊髓能量代谢障碍。

Objective：To assess the changes of energy metabolism and NF-κBp65 during spinal cord ischemia and reperfusion injury（SCIRI）. Method：The SCIRI model was made by clamping the infrarenal aortic for 45 minutes in 40 rabbits.Reactive oxygen species（ROS）,the expression of nuclear factor-kappa Bp65（NF-κBp65）,inhibitor-Kappa Bα（I-κBα）,intercellular adhesion molecule（ICAM-1）and the content of adenine nucleotide（ADP,AMP and ATP）in spinal cord tissue were determined before ischemia,45 minutes after ischemia,30,60 minutes and 120 minutes after reperfusion. Result：The spinal cord content of adenine nucleotide and I-κBαdecreased while ROS,the expressions of NF-κBp65and ICAM-1 increased at 45 minutes after ischemia,30,60 minutes and 120 minutes after reperfusion as compared to the value before ischemia（P〈0.01）. Conclusion：The upregulation of the expression of ICAM-1 and NF-κBp65 aggravates the energy metabolism disorder of spinal cord during SCIRI.