一起由宋内志贺菌引起食物中毒的病原菌检测分析

Detection and analysis of pathogen in a food poisoning case caused by Shigella sonnei

目的通过实验室检测分析一起食物中毒的病原菌。方法按GB/T 4789.36—2008、GB 4789.4—2010、GB4789.10—2010、GB 4789.5—2012《食品安全国家标准食品微生物学检验》中的方法,对24份患者吃剩的食物、12份患者大便进行肠出血性大肠埃希菌O157∶H7、沙门菌、金黄色葡萄球菌、志贺菌的分离培养及生化鉴定;同时采用多重PCR检测技术对24份患者吃剩的食物进行以上项目的检测。并对检出的志贺菌菌株进行血清凝集试验、药敏试验和脉冲场凝胶电泳(PFGE)分子分型。结果 24份患者吃剩的食物中检出宋内志贺菌1株,12份患者大便中检出宋内志贺菌8株,9株宋内志贺菌PFGE基因图谱相似性系数≥0.98。结论由宋内志贺菌Ⅰ相导致了这起食物中毒的发生。建议各级卫生监督部门要加大监督检查力度,从而减少食物中毒的发生,切实保障儿童身体健康。

Objective To investigate the causative pathogen of a food poisoning by laboratory analysis. Methods According to the method in GB / T 4789. 36—2008,GB 4789. 4—2010,GB 4789. 10—2010,GB 4789. 5—2012 ＂National food safety standard- food microbiological examination＂,24 patients＇ leftover foods and 12 patients＇ feces,all specimens were collected to detect enterohemorrhagic Escherichia coli O157： H7,Salmonella,Staphylococcus aureus and at the same time,using multiplex PCR detection technology detected 24 patients＇ leftover foods for the above project. These Shigella strains were analyzed using the methods of serological tests,drug sensitive tests and pulsed field gel electrophoresis. Results 1 strains of Shigella was sonnei were detected out from 24 leftover foods and 8 strains of Shigella sonnei were detected out from 12 patients＇ feces. Similarity coefficient of the 9 strains Shigella sonnei＇s PFGE genetic is greater than or equal to 0. 98. Conclusion This food poisoning was caused by Shigella sonnei phase I. It is suggested that the health administrative department at each level should strengthen the supervision and inspection to reduce the occurrence of food poisoning and ensure the children＇s health.