IL-17在哮喘小鼠模型中表达及其对肥大细胞IL-6分泌的影响

Expression of IL-17 in asthmatic mice and its eftect on release of IL-6 in mast cells

目的 :研究白细胞介素(interleukin,IL)-17在哮喘小鼠模型中的表达,观察其对肥大细胞IL-6分泌的影响。方法:BALB/c小鼠随机分为对照组和哮喘组,分别用等量磷酸盐缓冲液(phosphate buffer solution,PBS)和卵清蛋白(ovalbumin,OVA)干预,检测肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中细胞总数及嗜酸性粒细胞(eosnophils,EOS)数验证模型的成功建立。应用逆转录-聚合酶链反应法、酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测两组小鼠肺组织中IL-17m RNA、BALF及血清中IL-17的表达差异。细胞实验分为对照组和IL-17组,分别予等量培养基和IL-17干预小鼠肥大细胞株(P815)后收集上清,用ELISA检测IL-6的水平。结果 :哮喘组BALF中细胞总数及EOS计数较对照组显著升高(P<0.05),模型建立成功。哮喘组肺组织中IL-17 m RNA、BALF及血清中IL-17表达较正常组显著升高(P<0.05)。细胞水平的研究发现IL-17组P815上清中IL-6表达较正常组显著升高(P<0.05)。结论 :IL-17在OVA诱导哮喘小鼠模型中表达升高。IL-17刺激肥大细胞分泌IL-6,可能在哮喘的发病中起促进作用。

Objective：To observe the expression of interleukin（IL）-17 in asthmatic mice and to investigate the effect of IL-17 on release of IL-6 in mast cells. Methods：BALB/c mice were randomly divided into control group and asthma group,and treated with equal amount of phosphate buffer solution（PBS）and ovalbumin（OVA）,respectively. Mouse asthma model was validated by detecting the total number of cells and cosinophils（EOS）in bronchoalveolar lavage fluid（BALF）. The expression differences between IL-17 mRNA in lung tissues and IL-17 in BALF and serum were measured by reverse transcription-polymerase chain reaction（RT-PCR）and enzyme-linked immunosorbent assay（ELISA）in the two groups. Mouse P815 mast cells were divided into the control group and the IL- l7 group, and treated with equal amount of culture medium and IL-17, respectively. The levels of released IL-6 in supernatants were detected by ELISA. Results：In BALF,the total number of cells and EOS in the asthma group were significantly increased than those in the control group（P 〈 0.05）,and the model was validated. The expressions of IL-17 mRNA in lung tissues and IL-17 in BALF and serum were significantly increased in the asthma group than those in the control group （P 〈 0.05）. In P815 cell supernatants,the secretion of IL-6 in the IL-17 group was significantly increased than that in the control group（P 〈 0.05）. Conclusion：The expression of IL-17 was increased in OVA-induced mouse asthma model. IL-17 may promote the process of asthma by stimulating IL-6 release by mast cells.