脂蛋白相关磷脂酶A2基因I198T和V279F多态性与冠状动脉粥样硬化病变程度的相关性

Association of I198T and A379V polymorphisms in lipoprotein-associated phospholipase A2 with extent of coronary atherosclerosis

目的 :探讨脂蛋白相关磷脂酶A2(Lp-PLA2)基因I198T、V279F多态性与冠状动脉粥样硬化病变严重程度和稳定性的相关性。方法:纳入268例冠状动脉粥样硬化患者及113例冠脉造影完全正常者,应用DNA测序仪检测Lp-PLA2基因I198T和V279F多态性,并进行统计学分析。结果:按照Gensini积分及冠心病临床分型,所分亚组的I198T和V279F基因型频率及其等位基因频率与对照组无差异;单支冠脉病变者IT+TT基因型和VF+FF基因型及其等位基因F频率高于对照组,而多支病变者则与对照组无差异。亦未发现I198T和V279F不同基因型在血脂水平及高血压、糖尿病患者比例上有差别。结论:未能发现Lp-PLA2基因I198T和V279F变异与冠状动脉粥样硬化病变严重程度和稳定性的显著相关性。

Objective：To investigate whether I198T and A379V polymorphisms in lipoprotein-associated phospholipase A2 （Lp- PLA2）correlate with severity and stability of coronary atherosclerosis. Methods：V279F and A379V polymorphisms in Lp-PLA2 gene of 268 patients with coronary atherosclerosis and 113 controls without coronary atherosclerosis were genotyped by DNA sequencing instrument and analyzed by statistical methods. Results：Stratified by Gensini score and clinical types, no significant associations were observed between subgroups and the controls in I198T and V279F genotypes and allele frequencies. As for subgroups stratified by numbers of diseased coronary branches,only subjects with one diseased coronary branches carried higher frequencies of genotype IT＋ TF,VF＋FF and F allele as compared to the controls. No associations were observed between patients with multi-vessel lesions and controls in I198T and V279F. In the further analysis of risk factors,blood fat levels and proportions of diabetes and hypertension patients in subjects carrying IT＋TT and VF＋VV genotypes showed no difference with II and VV genotypes. Conclusion：No associations existed between I198T and V279F polymorphisms in Lp-PLA2 and the severity and stability of coronary atherosclerosis.