沉积物燃料电池阳极区纤维素分解菌群筛选及酶学特性

Selection and characterization of microbial community with capacity degrading cellulose from anode of sediment microbial fuel cell

以沉积型微生物燃料电池(SMFC)的阳极区为筛选源,通过电化学的方法,经过对菌群的富集培养,分别从电极上、电极近端以及电极远端筛选得到3组纤维素分解混合菌群。通过对3组菌群滤纸降解特性的研究表明,距离电极越近,滤纸酶活活性越高,最高可达56.8 U,电极上的菌群分解产物的含糖量是电极远端的含糖量1.67倍,3组菌群的酶反应环境偏酸性,耐热性能比较好;距离电极越近,对Ca2+浓度的耐受性越强,Mg2+对酶促反应有促进作用,由此可以看出,距离电极越近,其降解纤维素能力越高。

With the electrochemical method and enrichment of culture,three microbial communities were selected from anode of sediment microbial fuel cell（SMFC）,which was located on carbon felt electrode,near-end of carbon felt,and far-end of carbon felt,respectively,and their cellulose enzyme properties were investigated.Results demonstrated that the closer distance to carbon felt electrode,the higher activity of filter enzyme activity.The highest filter paper enzyme activity could reach 56.8 U.The sugar content of decomposition product on carbon felt electrode was 1.67 times as that of the distant end.Their enzyme reaction environment tended to acidity and had good heat resistance.In addition,for the enzyme reaction,the closer distance to carbon felt electrode,the more resistance of Ca2＋,while the Mg2＋ could promote the enzyme reaction.Results showed that the closer distance to carbon felt electrode,the better of degradation of cellulose.