青海省互助、泽库和祁连县啮齿类动物伯氏疏螺旋体调查

Investigation of Borrelia burgdorferi in Glires in Huzhu, Zekog,and Qilian county, Qinghai

目的调查确定青海省3县啮齿类标本中伯氏疏螺旋体带菌情况。方法从青海省互助、泽库和祁连县共采集啮齿类动物202只,采用巢式PCR方法检测其伯氏疏螺旋体的带菌率。结果采用巢式PCR方法检测啮齿类动物标本202份,阳性49份,伯氏疏螺旋体平均阳性率为24.26%。其中互助县标本共67份,阳性12份,阳性率为17.91%;泽库县标本共78份,阳性27份,阳性率为34.62%;祁连县标本共57只,阳性10份,阳性率为17.54%。3个县的啮齿类动物伯氏疏螺旋体阳性率差异有统计学意义(χ2=7.40,P<0.05)。结论青海省互助、泽库和祁连县啮齿类动物中均存在伯氏疏螺旋体感染,应进一步对当地的媒介和人群进行调查,从而为当地莱姆病的防控提供参考。

Objective To identify the Borrelia burgdogreri in Glircs in Huzhu, Zekog, and Qilian county, Qinghai province. Methods A total of 202 Glires were collected from forest in the counties, Qinghai province. All the collected samples were examined forB. burgdogferi by nested PCR. Results In total of 202 samples, 49 samples were tested positive by 24.26%. Positive rate was 17.91% （12/67） in Huzhu county, 34.62% （27/78） in Zekog county and 17.54% （10/57） in Qilian county. There was significant difference with positive rate of B. burgdorferi in Glires among these three counties（χ2=7.dO, P〈0.05）. Conclusion Our research confirmed the existence of B. burgdoTferi in Glires in three counties in Qinghai province. We suggested that the further investigation on local vectors and human infections be conducted for prevention and control of Lyme disease.