大孔树脂分离纯化葡萄籽原花青素的研究

Seperation and enrichment of anthocyanin from grape seed by macroporous resin column chromatography

本文研究了大孔树脂分离纯化葡萄籽提取物中原花青素的方法。先对5种大孔吸附树脂用静态筛选方法选择HPD100和HPD400树脂,进一步以动态吸附与解吸附实验比较了两种树脂对葡萄籽提取物原花青素的吸附与洗脱效果。结果表明:HPD100、HPD400树脂对葡萄籽提取物中原花青素的动态饱和吸附量分别为101.2和92.1mg·g-1,乙醇洗脱的总洗脱率分别为96.5%和92.0%;其中30%乙醇洗脱样品中原花青素含量分别为74.8%和85.0%;50%乙醇洗脱样品中原花青素含量分别为70.0%和73.9%。与HPD100相比较,HPD400更适合葡萄籽原花青素的分离与富集。

The method for seperation and purification of anthocyanin from grape seed by macroporous resin column chromatography were studied in this work. Firstly, five kinds of macroporous resin were screened by static screening technology. Then resin HPD 100 and resin HPD 400 were choosed out as adsorbent of dynamic seperation. The results indicated that adsorptive capacity of rensin HPD100 and HPD 400 are 101.2 and 92.1mg·g^-1 with the elution rate of 96.5% and 92.0%, respectively. The content of anthocyanin in samples eluted by 30% ethanol were 74.8% and 85.0% and the content of anthocyanin in samples eluted by 50% ethanol 70.0% and 73.9%. Compared with resin HPD 100, HPD 400 is more effective for seperation and enrichment of anthocyanin from grape seed.