克百威分子印迹固相萃取微球的制备及性能

Preparation and Characteristic of Carbofuran Molecularly Imprinted Polymer Microspheres for Solid-Phase Extraction

以偶氮二异丁腈为引发剂,2-3μm的聚苯乙烯微球为种球,克百威为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用单步溶胀法制备粒径均一的克百威分子印迹聚合物微球（MIPMs）。通过扫描电镜（SEM）、吸附平衡实验和竞争吸附实验分析了克百威MIPMs的形貌及其对克百威的结合特性及吸附选择性,并比较了克百威分子印迹固相萃取柱（MISPE）与C18固相萃取柱（C18SPE）富集水中克百威的效果。结果表明：合成的MIPMs粒径约10μm,表面呈蜂窝状;在90min内可达到饱和吸附,最大吸附量为25.94mg/g;在克百威、灭多威和三羟基克百威共存的条件下,克百威MIPMs可实现对克百威的专一性吸附;与C18SPE相比,克百威MISPE重复使用6次后加标回收率仍在85%以上,可用于水体中痕量克百威的检测。

Uniform-sized carbofuran molecularly imprinted polymer microspheres（MIPMs）were prepared by one-step swelling method using azobisisobutyronitrile as initiator,2—3μm polystyrene microspheres as seed,carbofuran as template,methacrylic acid as functional monomer,ethylene glycol dimethacrylate as cross-linker.The Scanning Electron Microscope（SEM）,equilibrium binding experiment and competitive adsorption experiment were used to study MIPMs＇ morphology,binding ability and adsorption selectivity.Enrichment effects of MISPE and C18 SPE were also compared.Results showed that the maximum binding capacity of carbofuran MIPMs was 25.94mg/g within 90min;these carbofuran microspheres could adsorb carbofuran selectively when carbofuran,methomyl and 3-hydroxycarbofuran coexisted in water.Compared to C18SPE＇s recovery,the carbofuran MISPE＇s recovery of samples spiked with carbofuran was above85% after using six times.It could be used to enrich carbofuran in water sample.