猫爪草皂苷对高转移肝癌细胞株HCCLM3及MHCC97-H增殖的影响

Study on the inhibition of extracts in Radix Ranunculi Ternati on cell HCCLM3 and MHCC97-H of liver cancer metastasis

目的观察猫爪草皂苷(RRTS)对人高转移肝癌细胞株HCCLM3、MHCC97-H增殖的影响。方法将对数生长期HCCLM3、MHCC97-H细胞分为两组。RRTS组分别采用50、100、200、400μg/m L浓度的RRTS干预,每浓度5个复孔;5-FU组采用50μg/m L的5-FU干预,作用后72 h采用MTT法测定两组细胞增殖情况,倒置显微镜下观察细胞形态变化。结果 RRTS组各浓度对HCCLM3、MHCC97-H肿瘤细胞株的生长均有不同程度的抑制作用,随RRTS浓度增加,抑制作用明显增强,呈量—效依赖关系(P均<0.05);400μg/m L对两种细胞的生长抑制率与5-FU组比较差异无统计学意义。倒置显微镜下观察,两种细胞株经RRTS处理后,细胞密度降低,细胞相互分离,贴壁脱落,出现片状无细胞生长区,并见细胞碎片,细胞变圆,体积减小,核固缩,核颜色加深,折光性增强,随着RRTS剂量增加,这种变化更加明显。400μg/m L浓度的RRTS对肿瘤细胞的生长抑制作用与5-FU组相似。结论RRTS对HCCLM3、MHCC97-H细胞生长均有抑制作用。

Objective To study the effect of the saponins on the HCCLM 3 and MHCC97-H proliferation of liver canc-er metastatic cells .Methods HCCLM3 and MHCC97-H of high metastatic hepatocellular carcinoma cell lines were used as the study object to detect the impact of concentrations of the saponins on the growth of liver cancer cells by MTT and a phase-contrast microscope was used to observe the impact of the saponins on hepatoma cell morphology .Results The cell survival rate of the saponins treatment group reduced compared with that of the control group .This effect becomes more ob-vious with the increasing concentration of the drug .The cell density reduced after the treatment of the saponins through the observation under the Phase-contrast microscope .Conclusion The saponins has an inhabitation effect on the growth of HCCLM3 cells and MHCC97-H cells.