蒙桑肌动蛋白基因的克隆与序列测定

Cloning and Sequence Analysis of Morus mongolica Actin Gene

为蒙桑基因表达与调控模式的研究以及蒙桑资源的利用提供参考,进行了蒙桑肌动蛋白(actin)基因的克隆与序列测定。结果表明:蒙桑actin基因mRNA序列大小为1 699bp,其中基因编码区1 134bp(GenBank登录号:KF031062),编码的377个氨基酸残基与其他植物的一致性在98%以上。该基因和川桑基因组中其他4个actin基因外显子数量和长度相同,但内含子差异极大,其CDS与桑树Morus010751(EXC30503)的一致性达到98%,氨基酸一致性为100%。聚类分析将其归为Class II类,与来自桑属和毛果杨的actin基因最先聚合在同一分支。

To provide a reference for gene expression and regulation mode in M.mongolica and M.mongolica resources utilization,the cloning and sequence analysis of M.mongolica actin gene was conducted.Results：Length of actin mRNA and CDS are respectively 1 699 bp and 1 134 bp （GenBank accession number：KF031062）.Homology analysis of the deduced 377 amino acid residues shows above 98% identity with that of other plants.There are the same exon number and length among this gene and the other three actin genes from mulberry genome,but varies obviously in introns.There are 98% identity between this gene and the Morus010751 （EXC30503）of mulberry in CDS,100% identity in amino acid. Clustering analysis showed that this gene is in Class II group,and the actin genes from mulberry and P .trichocarpa are in the same branch