普安银鲫成熟卵及受精后FAS和ACC的表达

FAS and ACC Gene Expressions in Mature Egg and Embryo after Fertilization of Carassius auratus

为探明普安银鲫雌鱼不同发育时期与脂代谢相关的脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACC)浓度及相对表达量的变化,以了解胚胎早期在脂质合成代谢方面的特点和规律性,采用酶联免疫分析法和实时荧光定量PCR法,测定成熟卵、受精卵、囊胚期(卵裂期胚胎)的FAS和ACC代谢水平及基因表达情况。结果表明:普安银鲫雌鱼成熟卵、受精卵和囊胚期均存在FAS和ACC,FAS含量在囊胚期显著升高(P<0.05),达到(2.48±0.31)nmol/L;ACC含量上升但差异不显著(P>0.05)。普安银鲫成熟卵、受精卵和囊胚期中均存在FAS和ACC基因表达,相对表达量逐渐增高,但差异均不显著(P>0.05)。

To explore the variation of concentration and relative transcript level of fatty acid synthetase （FAS）and Acetyl CoA carboxylase （ACC）in different stages of C.auratus,so as to know the features and regularity of lipid anabolism in early stage of embryo,both the enzyme concentration and mRNA expression level of FAS and ACC were determined in mature egg and embryo after fertilization by ELISA and real-time PCR.Results：Enzyme concentration existed in mature egg,fertilized egg and blastula stage embryo.FAS enzyme concentration increased to （2.48 ± 0.31 ）nmol/L （P 〈0.05 ）,ACC enzyme concentration increased gradually （P 〉0.05）.FAS and ACC gene expressed in mature egg,fertilized egg and blastula stage embryo,relative transcript level increased gradually（P 〉0.05）.