转merA基因金盏菊对环境中汞污染的修复效果

Remediation Effect of Transgenic Calendula officinalis with merA Gene on Mercury Pollution

为了提高金盏菊植物对环境中重金属(Hg)污染的修复效果,构建merA重组表达载体质粒,再经农杆菌介导法建立遗传转化体系筛选出转merA基因的金盏菊植株,研究其对汞的富集率。结果表明:转merA基因金盏菊对土壤中Hg2+的转移率提高84%,而且大部分Hg2+在merA基因作用下,转化为无毒的Hg挥发到空气中,提高了其忍受HgCl2的浓度;在Hg2+胁迫下,转基因植株中脯氨酸含量、SOD酶活性、POD酶活性及MDA含量均有不同程度的提高。转基因金盏菊具有较强的抗重金属汞胁迫的能力。

The remediation effect of transgenic C.officinalis with merA gene obtained by Agrobacterium-mediated genetic transformation system on mercury pollution was analyzed to study the mercury enrichment rate.The results showed that the 84% Hg2＋ in soil can be transported into leaves of transgenic C.officinalis with merA gene and then most Hg2＋ in leaves can be converted into nontoxic Hg under the action of merA gene,which can increase its tolerance to HgCl2 stress.The proline and MDA content,and activity of SOD and POD of transgenic C.officinalis with merA gene are increased under Hg2＋ stress to varying degrees.In conclusion,transgenic C.officinalis with merA gene has the higher tolerance to Hg2＋ stress.