不同抑制剂脱除方法对玉米秸秆水解液发酵的影响

Comparison of Detoxification Methods of Corn Stover Hydrolysate for Butanol Production

通过向玉米秸秆水解液中添加活性炭、Ca(OH)2、活性炭+Ca(OH)2、亚硫酸钠和连二亚硫酸钠五种物质,研究了不同抑制剂脱除方法对水解液各组分及其对发酵的影响。结果表明,抑制剂脱除率由高到低依次为:活性炭+Ca(OH)2>活性炭>Ca(OH)2>亚硫酸钠和连二亚硫酸钠,这与对还原糖造成的损失一致。对Clostridium beijerincii 8052发酵水解液的影响,由好到差依次为:Ca(OH)2>活性炭>活性炭+Ca(OH)2>亚硫酸钠和连二亚硫酸钠。活性炭+Ca(OH)2的组合虽能最大程度去除糠醛、羟甲基糠醛和可溶性木素三种抑制剂,但从发酵结果来看,脱除抑制剂后的水解液中还存在其它未知组分对厌氧发酵产生一定影响。

By adding the following five substances to corn stover hydrolyzate： charcoal, Ca（OH）2, charcoal and Ca（OH）2, sodium sulfite, and sodium hydrosulfite, studied the effects of different methods of inhibitor removal on the components in hydrolyzate and in the process of fermentation. The results showed that the removal rate of inhibitors was in descending order： charcoal ＋ Ca（OH）E, charcoal, Ca（OH）2 , sodium sulfite and sodium hydrosulfite, which was consistent with the loss of reducing sugar. While removal factors of the detoxified hydrolyzate fermented with Clostridium beijerincii 8052 were rated from high to low： Ca（OH）E, charcoal, charcoal ＋ Ca（OH）2, sodium sulfite and sodium dithionite. Although method of charcoal ＋ Ca（OH）2 achieved the greatest removal of furfural, HMF and soluble lignin, as the fermentation research findings showed was found that some unknown components in the hydrolyzate still have a bad impact on the anaerobic fermentation.