混合藻中蓝藻的检测

Detection of cyanobacteria in mixed algae

利用荧光光谱技术,以铜绿微囊藻活体、藻蓝蛋白提取液以及叶绿素a提取液为测试对象,进行了荧光光谱的扫描,并且研究了不同藻种对铜绿微囊藻藻蓝蛋白荧光强度的影响。研究结果表明,测定铜绿微囊藻的检测波长为590nm/650nm,铜绿微囊藻的细胞密度随荧光强度的增大而增大,呈正相关关系,r=0.999 4。

With the help of fluorescence spectra, Microcystis aeruginosa in vivo, phycoeyanin extracting solution and chloro- phyll a extracling solution are investigated by using fluorescence quenching method, and the influence of different algal species to fluorescence intensity of microcystis aeruginosa phycocyanin is studied. The research results show that, the detection wave- length for determination of Microcystis aeruginosa is 590 nm/650 nm, the cell density of Microcystis aeruginosa and algal pho- tosynthetic signal have significant positive correlation, and r=0. 999 4.