CARTp对脑缺血再灌注小鼠神经功能的影响

Effect of CARTp on neuro-function after cerebral ischemia-reperfusion

目的探讨可卡因-苯丙胺调节转录肽(CARTp)对不同时间段脑缺血再灌注小鼠神经功能的影响。方法将成年美国癌症研究所雄性小鼠随机分为三组:假手术组(Sham组)、大脑中动脉阻塞组(MCAO组)和CARTp处理组,每组15只。采用线栓法复制MCAO模型。CARTp处理组小鼠于缺血再灌注第2天经尾静脉注射CARTp(2.5μg/kg),以后每天注射2.5μg/kg至第10天;Sham组、MCAO组注射生理盐水。通过神经功能评分判断神经功能障碍程度。观察并比较三组小鼠脑缺血再灌注损伤后的神经功能评分,判断不同处理组小鼠的神经功能障碍情况。结果 MCAO组和CARTp处理组神经功能评分均显著高于Sham组,差异均有统计学意义(P<0.05);CARTp处理组的神经功能评分从第1天至第8天均低于MCAO组,差异均有统计学意义(P<0.05)。结论 CARTp能减轻小鼠脑缺血再灌注损伤引起的神经功能障碍,并可能在脑神经功能恢复中发挥重要作用,但随着时间延长,CARTp减轻神经功能障碍的作用明显减弱。

Objective To explore the effect of Cocaine—and amphetamine—regulated transcript（CARTp） on mice′s neuro—function after cerebral ischemia—reperfusion at different time periods. Methods Adult male Institute of Cancer Research （ICR） rats were randomly divided into the Sham operation group（the Sham group）,the middle cerebral artery occlusion group（MCAO group） and the CARTp group,15 of each group. The transient middle cerebral artery occlusion （MCAO） rat model was per—formed by suture—occluded method. The 2.5μg/kg CARTp was injected to the mice in the CARTp group by vena caudalis on sec—ond day after MCAO and then 2.5 μg/kg daily till the tenth day while the Sham group and the MCAO group with normal saline. Neurological deficits degree were evaluated by Bederson′s score. It was observed and compared the neurological deficits after cerebral ischemia—reperfusion injury to judge the neurological deficits of the three rats groups. Results The neurological score of the MCAO group and the CARTp group was significantly higher than that of the Sham group ,which was statistically significant in difference （P〈0.05）. The neurological score of the CARTp group from the first day to the eighth day was lower than that of the MCAO group. The difference had statistical significance （P〈0.05）. Conclusion CARTp may relieve the neurological deficits af—ter cerebral ischemia—reperfusion,and also plays an important role in recovery of brain—neural functions.