摘要
对腐乳中桔霉素提取条件进行优化,确定最佳提取条件为甲苯—乙酸乙酯—甲酸(7∶3∶1,V/V)超声处理10min,提取温度50℃,提取3次,提取液经浓缩后甲醇定容。建立高效液相色谱荧光检测器法测定腐乳中桔霉素,采用Hypersil ODS 2(250 mm×4.6 mm,5μm)为分析柱,水(p H2.5)—乙腈(70∶30,V/V)为流动相,流速0.8 m L/min,采用荧光检测器分析,外标法定量。结果表明:桔霉素在0.01-100.00μg/m L范围内线性良好(R2=0.999 99),检出限0.005 mg/kg,样品加标回收率为90.4%-107.9%,相对标准偏差(RSD6)小于6%,该方法操作简便、结果可靠。
Extracted conditions for citrinin were optimized in sufu.Citrinin was extracted completely by toluene-ethyl acetate-formic acid(7 ∶ 3 ∶ 1,V / V) at 50 ℃,with 10 min ultrasonic processing.A method for determining citrinin in sufu was established by high performance liquid chromatography(HPLC) with fluorescence detection.Citrinin was separated by mobile phase of water(p H 2.5)-acetonitrile(70∶ 30) on Hypersil ODS2 column.The results were showed that there was good linear relationship between the fluorescence detection values and citrinin concentration in the range of 0.01 - 100.00 μg / m L(R2= 0.999 99).The limit of detection was 0.005 mg / kg and the recovery of citrinin were between 90.4% and107.9% and relative standard deviation(RSD,n = 6) value were less than 6%.
出处
《食品与机械》
CSCD
北大核心
2015年第2期102-105,共4页
Food and Machinery
基金
建德市科技项目(编号:2011C17)