斗鸡Nramp1基因多态性与部分免疫指标相关性分析

Association Analysis between Polymorphisms of Nramp1 Gene and Immune Indexes in Game Chicken

随机选取同期孵化出雏的漳州斗鸡32只,测定12周龄鸡的部分免疫指标。利用PCR-SSCP技术检测斗鸡Nramp1基因第九外显子的多态性,并对不同基因型与免疫指标进行相关分析。结果显示:斗鸡Nramp1基因第九外显子在C315G、C357T处有2个突变,两处均为同义突变,并检测到3种基因型,不同基因型在异嗜性细胞与淋巴细胞比率(H/L值),Ig M和Ig M含量均呈现出显著差异(P<0.05)。AA基因型的H/L值、Ig G和Ig M含量均显著高于BB基因型,Ig M和Ig G含量与AB型差异显著(P<0.05),其余指标与AB基因型差异不显著。初步研究结果显示,斗鸡AA基因型的综合免疫性能要优于AB和BB型,可作为一种高抗性基因型。

Thirty-two Zhangzhou game chicken（DJ）were randomly selected and incubated at the same stage. Some immune indexes of chickens at 12 weeks of age were detected. PCR-SSCP technique and sequencing method were applied to detect SNPs in exon 9 of Nramp1 gene,then analyze relationship between the polymorphisms of Nramp1 gene and immune indexes. The results showed that 2 samesense mutations at C315 G and C357 T loci were found in exon 9 of Nramp1 gene of DJ,and three genotypes were detected. The heterophil/lymphocyte（H/L）value,the content of Ig G and Ig M of different Nramp1 genotypes in DJ showed significant differences（P〈0.05）. H/L value,the content of Ig G and Ig M of AA genotype were significantly higher than those of BB genotype（P〈0.05）,the contents of Ig M and Ig G of AA were significantly different from that of AB genotype,while there were no differences between other immune indexes of AA and AB genotypes. The results of preliminary study revealed that the general immune performance of AA genotype was superior to that of BB and AB genotype,and could be used as high resistance genotype.