炎症介质IL-6及IL-8在撞击伤肺组织中的表达研究

Expressions of IL-6 and IL-8 in lung injuries induced by blunt chest trauma

目的探讨炎症介质白细胞介素(IL)-6及IL-8在声门紧闭与开放状态下闭合性胸部撞击所致肺损伤组织中的表达。方法采用RT-PCR法检测声门紧闭与开放状态下,不同时相点胸部撞击伤侧与对照组肺组织IL-6和IL-8mRNA的表达。结果伤后4、8、12h声门紧闭组IL-6和IL-8mRNA的表达显著高于声门开放组(P<0.05)。伤侧肺组织中IL-6和IL-8mRNA表达各时相点显著高于与对照组(P<0.05)。对照组、声门紧闭组和声门开放组的肺组织两种细胞因子IL-8和IL-6mRNA的表达变化规律相似,表达明显上调。结论提示IL-6和IL-8可能在肺撞击伤后肺脏功能损害的发生、发展过程中发挥重要的作用。

Objective To study the expression of IL-6and IL-8in the lung injuries induced by blunt chest trauma under the closure and open state of glottis.Methods The expressions of IL-6and IL-8mRNA were detected by RT-PCR in injuried lung tissues under the closed and open glottis states snd the control lung tissues at designed different time points.Results The expressions of IL-6and IL-8mRNA at 4,8,12 hin the closed glottis group were obviously higher than those in the open glttis group（P〈0.05）and the expressions at designed various time points in the injured lung tissue were higher than those in the control lung tissues（P〈0.05）.The change rule of IL-6and IL-8mRNA expression in the control group,closed glottis group and open glottis group was similar,the expression was up-regulated.Conclusion IL-6and IL-8might play an important role in the occurrence and development of functional lesion in the injured lung by blunt chest trauma.