不同海拔产地烟草种子的萌发及抗逆相关基因表达研究

Study on seed germination and stress-responsive gene expression of tobacco seedlings from different areas and altitudes

以不同海拔产地生产的MS K326烟草种子为材料,分析了室内发芽及胁迫响应途径的相关基因的表达水平和不同烟区苗期生长速率变化。结果表明,萌发4 d,低海拔产地的种子发芽比例最高,5 d后,各海拔产地的种子发芽比例基本一致。萌发7 d,不同海拔产地生产的烟草种子的抗氧化系统、渗透调节、转录调节等关键基因表达水平有一定差异,14 d时,差异变小,到21 d,无明显差异。在6个烟叶产区漂浮育苗时,不同海拔产地生产的烟草种子,其出苗时间略有不同,但成苗时间一致。因此,不同产地海拔条件下生产的烟草种子,其发芽比例及抗性相关基因表达仅在发芽14 d前略有不同,之后没有差异。

Seed germination rate,stress-related gene expression and seedling growth rate in tobacco seeds of MS K326 were determined,which produced in different areas with different altitudes. The results indicated that seed germination percent of tobacco from low altitude was the highest at 4thday. After 5thday,there was no significant difference in germination percent of seeds produced from different altitudes. Differences in expression levels of genes involved in multiple stress-responsive pathways,including antioxidant system,osmotic adjustment and transcription factors were observed at 7thday,and then the difference decreased at 14 thday,and there was no significant difference at 21 stday. In six production areas,the seedling emergency time were a little different among the tobacco seeds from different altitudes,but the seedling time were the same. Accordingly,the germination percent and stress-related geneexpression of MS K326 seeds,which produced in different altitudes,showed no significant difference after 14 days germination.