利用基因功能性标记检测水稻稻瘟病和褐飞虱的抗性基因

Detecting the rice blast resistance genes and brown planthopper resistance genes by gene functional marker

选育和利用水稻抗性品种,是防治稻瘟病和褐飞虱的有效方法。利用基因功能性标记对2个优质不育系和16个恢复系的抗性基因Pi-b,Pi-k,Pi-ta,bph2,Bph3进行检测,结果 11份材料含有Pi-b基因,13份含有Pi-k基因,9份同时含有Pi-b基因和Pi-k基因,3份含有bph2基因。通过对抗性基因的分子鉴定,为多基因聚合、选育广谱持久抗稻瘟病和褐飞虱的优良品种奠定基础。

Breeding and utilizing resistant rice cultivars is an effective method for controlling rice blast and brown planthopper. The rice blast resistance genes Pi-b,Pi-k,Pi-ta and brown planthopper resistance genes bph2,Bph3 of 2male sterile lines and their 16 restorer lines were detected by gene functional marker. The results showed that 11 tested materials contained gene Pi-b,13 tested materials contained gene Pi-k,9 tested materials contained both gene Pib and Pi-k,and 3 tested materials contained gene bph2. It laid the foundation for the gene pyramiding and breeding the excellent rice cultivars with broad spectrum and durable resistance to rice blast and brown planthopper by molecular identification to related resistance genes of rice cultivars.