己糖激酶-Ⅱ在直肠癌组织中表达临床意义

The clinical value of Hexokinase-Ⅱ expression in carcinoma of the rectum

目的:检测己糖激酶-Ⅱ基因在直肠癌组织中表达,探讨其与患者预后的关系。方法:通过逆转录聚合酶链反应(RT-PCR)、蛋白质印迹方法、免疫组织化学方法了解己糖激酶-Ⅱ在直肠癌100例组织中表达,探讨与肿瘤病理因素关系。结果:RT-PCR和蛋白质印迹法结果显示直肠癌组织阳性表达己糖激酶-Ⅱ在RNA水平显著高于正常组织阳性表达。直肠癌组织中己糖激酶-Ⅱ表达阳性率显著高于对照组(P<0.01)。肿瘤大小、分化程度、合并肿瘤分期与己糖激酶-Ⅱ的表达呈正相关(P值分别为P=0.001,P=0.000,P=0.019),而淋巴转移与否与己糖激酶-Ⅱ的表达无相关性(P=0.815)。结论:直肠癌组织中HK-Ⅱ呈高表达,HK-Ⅱ抑制剂能抑制肿瘤糖酵解从而抑制肿瘤生长、增殖,促进肿瘤凋亡,为肿瘤治疗提供契机。

Objective：To study the expression of Hexokinase-II in carcinoma of the rectum,and analyze the relation-ships between HK-II expression and the clinical features. Methods： The expression of HK-II in 100 carcinoma of the rec-tum tissue was checked by using reverse transcriptase polymerase chain reaction,Western blot and inununohistochemistry technique.Then the relationships between HK-II expression and the clinical features of carcinoma of the rectum patients were further investigated,such as tumor sizes,clinical stages,differentiation levels,lymphatic metastasis. Results：RT-PCR re-sult showed that positive expression HK-II in carcinoma of the rectum tissue were higher than that in normal tissue on RNA level.Western blot result showed that positive expression HK-II in carcinoma of the rectum tissue were higher than that in normal tissue on protein level.Inununohistochemistry results showed that the cancer tissue had more positive expres-sion than normal rectal mucosa tissue（P=0.000）.Inununohistochemistry results showed that tumor sizes, differentiation lev-els and merger of tumor stages was related with positive expression HK-II （prespectively 0.001,0.000,0.019）, lymphatic metastasis was not related with positive expression HK-II （P=0.815）. Conclusions：The cancer tissue had more positive HK-II expression than normal rectal mucosa tissue. Tumor sizes,clinical stages and differentiation levels were related with positive expression of HK-II, lymphatic metastasis was not related with positive expression of HK-II.