肾病综合征大鼠血清IL-4和IL-12的动态变化及其意义

Dynamic changes and significance of serum levels of IL- 4 AND IL- 12 in rats of nephrotic syndrome

目的:本实验通过微小病变型肾病综合征模型(MCNS),观察血清IL-4、IL-12的动态变化,探讨MCNS的免疫学发病机制。方法:将40只大鼠随机分为正常组、肾病组,每组20只。阿霉素5mg/kg制MCNS模型。观察大鼠一般情况,检测24h尿蛋白、血清白蛋白和总胆固醇水平,用ELISA检测血清IL-4、IL-12的水平。结果:与正常组比较,肾病组出现大量的蛋白尿,低白蛋白血症,高胆固醇血症和明显的水肿,血清IL-4升高,IL-12降低。结论:1MCNS存在TH1/TH2功能紊乱,IL-4、IL-12参与了MCNS的免疫机制。

Objective： To observe developing changes of serum value IL- 12,IL- 4 in rats by using minimal change nephrotic syndrome（ MCNS）; and to discuss MCNS immunopathogenesis. Methods： Forty wistar rats were randomly divided into normal group,nephritic group with 20 rats in each group. MCN models were induced by adriamycin at a dose of 5mg / kg. Each group＇s normal conditions were observed. 24 h urinary protein,serum albumin and serum total cholesterone were measured. IL- 4,IL- 12 serum level were measured with ELISA Results： there were quantitative proteinuria,hyperlipidemia,hypoalbuminemia and evidence edema in nephritic group compared with normal group. the serum il- 4 level was higher,but serum IL- 12 level was lower in nephritic group than those in normal group. Conclusion： Th1 / Th2 functional imbalance exists in patients with MCNS. IL- 4 and IL-12 are involved in the immunologic mechanisms.